PDF(Pubmed)
Abstract：
UNASSIGNED: Our previous studies proved that neurogenic inflammatory spots (or neurogenic spots) have the same physiological features as acupuncture points and that neurogenic spot stimulation generates therapeutic effects in various animal models. However, it is unclear how deeply the neurogenic spots should be stimulated to generate therapeutic effects.
UNASSIGNED: The effects of acupuncture at various needle depths below the neurogenic spot were examined in a rat immobilization stress-induced hypertension (IMH) model. Electroacupuncture was applied to a neurogenic spot at depths of 1, 2, or 3 mm using a concentric bipolar electrode.
UNASSIGNED: Electrical stimulation of the neurogenic spot at a 3-mm depth most effectively lowered blood pressure compared with controls and stimulation at 1- and 2-mm depths, which was inhibited by pretreatment with a local anesthetic lidocaine. Electrical stimulation of the neurogenic spot or injection of substance P (SP) at a 3-mm depth significantly excited the rostral ventrolateral medulla (rVLM) compared with superficial stimulation. Electrical stimulation applied at a 3-mm depth on neurogenic spots dominantly caused c-fos expression from rVLM and ventrolateral periaqueductal gray (vlPAG) in IMH rats. Pretreatment with resiniferatoxin (RTX) injection into the neurogenic spot to ablate SP or calcitonin gene-related peptide (CGRP) prevented the effects of 3-mm neurogenic spot stimulation on blood pressure in IMH rats. Conversely, artificial injection of SP or CGRP generated anti-hypertensive effects in IMH rats.
UNASSIGNED: Our data suggest that neurogenic spot stimulation at a 3-mm depth generated anti-hypertensive effects through the local release of SP and CGRP and activation of rVLM and vlPAG.
UNASSIGNED: The effects of acupuncture at various needle depths below the neurogenic spot were examined in a rat immobilization stress-induced hypertension (IMH) model. Electroacupuncture was applied to a neurogenic spot at depths of 1, 2, or 3 mm using a concentric bipolar electrode.
UNASSIGNED: Electrical stimulation of the neurogenic spot at a 3-mm depth most effectively lowered blood pressure compared with controls and stimulation at 1- and 2-mm depths, which was inhibited by pretreatment with a local anesthetic lidocaine. Electrical stimulation of the neurogenic spot or injection of substance P (SP) at a 3-mm depth significantly excited the rostral ventrolateral medulla (rVLM) compared with superficial stimulation. Electrical stimulation applied at a 3-mm depth on neurogenic spots dominantly caused c-fos expression from rVLM and ventrolateral periaqueductal gray (vlPAG) in IMH rats. Pretreatment with resiniferatoxin (RTX) injection into the neurogenic spot to ablate SP or calcitonin gene-related peptide (CGRP) prevented the effects of 3-mm neurogenic spot stimulation on blood pressure in IMH rats. Conversely, artificial injection of SP or CGRP generated anti-hypertensive effects in IMH rats.
UNASSIGNED: Our data suggest that neurogenic spot stimulation at a 3-mm depth generated anti-hypertensive effects through the local release of SP and CGRP and activation of rVLM and vlPAG.
摘要：
■我们先前的研究证明，神经源性炎症斑点（或神经源性斑点）具有与穴位相同的生理特征，并且神经源性斑点刺激在各种动物模型中产生治疗作用。然而,目前尚不清楚神经源性斑点应受到多大程度的刺激以产生治疗效果。
■在大鼠固定应激诱发的高血压（IMH）模型中检查了在神经源性斑点下方的各种针深度处针刺的效果。使用同心双极电极将电针应用于1、2或3mm深度的神经源性斑点。
■与对照组和1毫米和2毫米深度的刺激相比，3毫米深度的神经源性点的电刺激最有效地降低了血压，用局部麻醉剂利多卡因预处理抑制。与浅层刺激相比，神经源性斑点的电刺激或3毫米深度的P物质（SP）的注射显着刺激了延髓腹侧延髓（rVLM）。在3毫米深度的神经源性斑点上施加电刺激主要引起IMH大鼠rVLM和腹外侧导水管周围灰色（vlPAG）的c-fos表达。用树脂毒素（RTX）注入神经源性斑点以消融SP或降钙素基因相关肽（CGRP）进行预处理，可防止3毫米神经源性斑点刺激对IMH大鼠血压的影响。相反，人工注射SP或CGRP对IMH大鼠产生降压作用。
■我们的数据表明，3毫米深度的神经源性斑点刺激通过SP和CGRP的局部释放以及rVLM和vlPAG的激活产生了抗高血压作用。
■在大鼠固定应激诱发的高血压（IMH）模型中检查了在神经源性斑点下方的各种针深度处针刺的效果。使用同心双极电极将电针应用于1、2或3mm深度的神经源性斑点。
■与对照组和1毫米和2毫米深度的刺激相比，3毫米深度的神经源性点的电刺激最有效地降低了血压，用局部麻醉剂利多卡因预处理抑制。与浅层刺激相比，神经源性斑点的电刺激或3毫米深度的P物质（SP）的注射显着刺激了延髓腹侧延髓（rVLM）。在3毫米深度的神经源性斑点上施加电刺激主要引起IMH大鼠rVLM和腹外侧导水管周围灰色（vlPAG）的c-fos表达。用树脂毒素（RTX）注入神经源性斑点以消融SP或降钙素基因相关肽（CGRP）进行预处理，可防止3毫米神经源性斑点刺激对IMH大鼠血压的影响。相反，人工注射SP或CGRP对IMH大鼠产生降压作用。
■我们的数据表明，3毫米深度的神经源性斑点刺激通过SP和CGRP的局部释放以及rVLM和vlPAG的激活产生了抗高血压作用。
