UNASSIGNED: The effects of acupuncture at various needle depths below the neurogenic spot were examined in a rat immobilization stress-induced hypertension (IMH) model. Electroacupuncture was applied to a neurogenic spot at depths of 1, 2, or 3 mm using a concentric bipolar electrode.
UNASSIGNED: Electrical stimulation of the neurogenic spot at a 3-mm depth most effectively lowered blood pressure compared with controls and stimulation at 1- and 2-mm depths, which was inhibited by pretreatment with a local anesthetic lidocaine. Electrical stimulation of the neurogenic spot or injection of substance P (SP) at a 3-mm depth significantly excited the rostral ventrolateral medulla (rVLM) compared with superficial stimulation. Electrical stimulation applied at a 3-mm depth on neurogenic spots dominantly caused c-fos expression from rVLM and ventrolateral periaqueductal gray (vlPAG) in IMH rats. Pretreatment with resiniferatoxin (RTX) injection into the neurogenic spot to ablate SP or calcitonin gene-related peptide (CGRP) prevented the effects of 3-mm neurogenic spot stimulation on blood pressure in IMH rats. Conversely, artificial injection of SP or CGRP generated anti-hypertensive effects in IMH rats.
UNASSIGNED: Our data suggest that neurogenic spot stimulation at a 3-mm depth generated anti-hypertensive effects through the local release of SP and CGRP and activation of rVLM and vlPAG.
■在大鼠固定应激诱发的高血压(IMH)模型中检查了在神经源性斑点下方的各种针深度处针刺的效果。使用同心双极电极将电针应用于1、2或3mm深度的神经源性斑点。
■与对照组和1毫米和2毫米深度的刺激相比,3毫米深度的神经源性点的电刺激最有效地降低了血压,用局部麻醉剂利多卡因预处理抑制。与浅层刺激相比,神经源性斑点的电刺激或3毫米深度的P物质(SP)的注射显着刺激了延髓腹侧延髓(rVLM)。在3毫米深度的神经源性斑点上施加电刺激主要引起IMH大鼠rVLM和腹外侧导水管周围灰色(vlPAG)的c-fos表达。用树脂毒素(RTX)注入神经源性斑点以消融SP或降钙素基因相关肽(CGRP)进行预处理,可防止3毫米神经源性斑点刺激对IMH大鼠血压的影响。相反,人工注射SP或CGRP对IMH大鼠产生降压作用。
■我们的数据表明,3毫米深度的神经源性斑点刺激通过SP和CGRP的局部释放以及rVLM和vlPAG的激活产生了抗高血压作用。