PDF(Pubmed)
Abstract：
Exhaled breath analysis, with particular emphasis on volatile organic compounds, represents a growing area of clinical research due to its obvious advantages over other diagnostic tests. Numerous pathologies have been extensively investigated for the identification of specific biomarkers in exhalates through metabolomics. However, the transference of breath tests to clinics remains limited, mainly due to deficiency in methodological standardization. Critical steps include the selection of breath sample types, collection devices, and enrichment techniques. GC-MS is the reference analytical technique for the analysis of volatile organic compounds in exhalates, especially during the biomarker discovery phase in metabolomics. This review comprehensively examines and compares metabolomic studies focusing on cancer, lung diseases, and infectious diseases. In addition to delving into the experimental designs reported, it also provides a critical discussion of the methodological aspects, ranging from the experimental design and sample collection to the identification of potential pathology-specific biomarkers.
摘要：
呼气分析,特别强调挥发性有机化合物，由于其明显优于其他诊断测试，因此代表了临床研究的增长领域。已经广泛研究了许多病理学以通过代谢组学鉴定呼出物中的特定生物标志物。然而,呼气测试向诊所的转移仍然有限，主要是由于方法标准化的不足。关键步骤包括选择呼吸样本类型，收集装置,和富集技术。GC-MS是分析呼气中挥发性有机化合物的参考分析技术，尤其是在代谢组学的生物标志物发现阶段。这篇综述全面审查和比较了专注于癌症的代谢组学研究，肺部疾病,和传染病。除了深入研究报道的实验设计，它还提供了方法论方面的批判性讨论，从实验设计和样品收集到潜在病理特异性生物标志物的鉴定。
