PDF(Pubmed)
Abstract：
Plant synthetic biology research requires diverse bioparts that facilitate the redesign and construction of new-to-nature biological devices or systems in plants. Limited by few well-characterized bioparts for plant chassis, the development of plant synthetic biology lags behind that of its microbial counterpart. Here, we constructed a web-based Plant Synthetic BioDatabase (PSBD), which currently categorizes 1677 catalytic bioparts and 384 regulatory elements and provides information on 309 species and 850 chemicals. Online bioinformatics tools including local BLAST, chem similarity, phylogenetic analysis, and visual strength are provided to assist with the rational design of genetic circuits for manipulation of gene expression in planta. We demonstrated the utility of the PSBD by functionally characterizing taxadiene synthase 2 and its quantitative regulation in tobacco leaves. More powerful synthetic devices were then assembled to amplify the transcriptional signals, enabling enhanced expression of flavivirus non-structure 1 proteins in plants. The PSBD is expected to be an integrative and user-centered platform that provides a one-stop service for diverse applications in plant synthetic biology research.
摘要：
植物合成生物学研究需要多种生物技术，以促进植物中新自然生物设备或系统的重新设计和构建。受植物底盘中很少有特征良好的生物艺术品的限制，植物合成生物学的发展落后于微生物。我们在这里构建了一个基于网络的植物合成生物学数据库(PSBD，https://www.bic.AC.cn/PSBD/front/#/)目前正在对1677催化生物制品进行分类，384个监管要素，以及309种和850种化学品信息。在线生物信息学工具，包括本地BLAST，化学相似性,提供了系统发育分析和视觉强度，以帮助合理设计遗传电路，以操纵植物中的基因表达。通过利用PSBD，进行了紫杉二烯合酶2（TcTS2）的功能表征及其在烟草叶片中的定量调控。组装了更强大的合成装置来放大转录信号，允许在植物中增强黄病毒非结构1（NS1）蛋白的表达。PSBD有望成为一个以用户为中心的综合平台，为植物合成生物学研究的各种目的提供一站式服务。
