PDF(Pubmed)
Abstract：
BACKGROUND: Chitosan is known to inhibit the growth of many bacteria and fungi. Tissue conditioners are commonly used to prevent bone destruction under dentures. However, over time, these materials can become a suitable substrate for microbial growth. One approach to improving dental materials is the use of nanoparticles. This study examined the antifungal properties of chitosan and green technique-synthesized silver nanoparticles in combination with tissue conditioners.
METHODS: Tissue conditioner materials were mixed with chitosan and silver nanoparticles at concentrations of 0.097%, 0.19%, and 0.37%, along with 1.25 ppm Nystatin, and their antimicrobial properties against Candida albicans were investigated. The growth rate was measured after 24 h of incubation at 37 °C. Non-parametric tests, such as the Kruskal-Wallis H test and Mann-Whitney U test with Bonferroni correction, were used for data analysis after verifying that the groups did not have a normal distribution.
RESULTS: Compared with the control and Nystatin groups, the Chitosan-silver groups showed a significant decrease in the number of CFUs of Candida albicans.
CONCLUSIONS: The combination of chitosan and silver nanoparticles with tissue conditioner materials is a promising alternative for preventing and treating denture stomatitis. These findings suggest that using very small amounts of nanoparticles in dental materials could effectively prevent microbial growth, which could improve the longevity and efficacy of dental prosthetics and materials.
METHODS: Tissue conditioner materials were mixed with chitosan and silver nanoparticles at concentrations of 0.097%, 0.19%, and 0.37%, along with 1.25 ppm Nystatin, and their antimicrobial properties against Candida albicans were investigated. The growth rate was measured after 24 h of incubation at 37 °C. Non-parametric tests, such as the Kruskal-Wallis H test and Mann-Whitney U test with Bonferroni correction, were used for data analysis after verifying that the groups did not have a normal distribution.
RESULTS: Compared with the control and Nystatin groups, the Chitosan-silver groups showed a significant decrease in the number of CFUs of Candida albicans.
CONCLUSIONS: The combination of chitosan and silver nanoparticles with tissue conditioner materials is a promising alternative for preventing and treating denture stomatitis. These findings suggest that using very small amounts of nanoparticles in dental materials could effectively prevent microbial growth, which could improve the longevity and efficacy of dental prosthetics and materials.
摘要：
背景:已知壳聚糖抑制许多细菌和真菌的生长。组织调理剂通常用于防止假牙下的骨破坏。然而,随着时间的推移,这些材料可以成为微生物生长的合适基质。改善牙科材料的一种方法是使用纳米颗粒。这项研究检查了壳聚糖和绿色技术合成的银纳米颗粒与组织调理剂的抗真菌性能。
方法：将组织调理剂材料与壳聚糖和银纳米颗粒混合，浓度为0.097％，0.19%，和0.37%，以及1.25ppm制霉菌素，并研究了它们对白色念珠菌的抗菌性能。在37°C孵育24小时后测量生长速率。非参数检验，如Kruskal-WallisH检验和带有Bonferroni校正的Mann-WhitneyU检验，在验证各组没有正态分布后，用于数据分析。
结果：与对照组和制霉菌素组相比，壳聚糖-银组显示白色念珠菌的CFU数量显着减少。
结论：壳聚糖和银纳米颗粒与组织调理剂材料的组合是预防和治疗义齿口炎的有希望的替代方法。这些发现表明，在牙科材料中使用非常少量的纳米颗粒可以有效地防止微生物生长，这可以提高牙科修复和材料的寿命和功效。
方法：将组织调理剂材料与壳聚糖和银纳米颗粒混合，浓度为0.097％，0.19%，和0.37%，以及1.25ppm制霉菌素，并研究了它们对白色念珠菌的抗菌性能。在37°C孵育24小时后测量生长速率。非参数检验，如Kruskal-WallisH检验和带有Bonferroni校正的Mann-WhitneyU检验，在验证各组没有正态分布后，用于数据分析。
结果：与对照组和制霉菌素组相比，壳聚糖-银组显示白色念珠菌的CFU数量显着减少。
结论：壳聚糖和银纳米颗粒与组织调理剂材料的组合是预防和治疗义齿口炎的有希望的替代方法。这些发现表明，在牙科材料中使用非常少量的纳米颗粒可以有效地防止微生物生长，这可以提高牙科修复和材料的寿命和功效。
