PDF(Pubmed)
Abstract：
CONCLUSIONS: The biostimulant Hanseniaspora opuntiae regulates Arabidopsis thaliana root development and resistance to Botrytis cinerea. Beneficial microbes can increase plant nutrient accessibility and uptake, promote abiotic stress tolerance, and enhance disease resistance, while pathogenic microorganisms cause plant disease, affecting cellular homeostasis and leading to cell death in the most critical cases. Commonly, plants use specialized pattern recognition receptors to perceive beneficial or pathogen microorganisms. Although bacteria have been the most studied plant-associated beneficial microbes, the analysis of yeasts is receiving less attention. This study assessed the role of Hanseniaspora opuntiae, a fermentative yeast isolated from cacao musts, during Arabidopsis thaliana growth, development, and defense response to fungal pathogens. We evaluated the A. thaliana-H. opuntiae interaction using direct and indirect in vitro systems. Arabidopsis growth was significantly increased seven days post-inoculation with H. opuntiae during indirect interaction. Moreover, we observed that H. opuntiae cells had a strong auxin-like effect in A. thaliana root development during in vitro interaction. We show that 3-methyl-1-butanol and ethanol are the main volatile compounds produced by H. opuntiae. Subsequently, it was determined that A. thaliana plants inoculated with H. opuntiae have a long-lasting and systemic effect against Botrytis cinerea infection, but independently of auxin, ethylene, salicylic acid, or jasmonic acid pathways. Our results demonstrate that H. opuntiae is an important biostimulant that acts by regulating plant development and pathogen resistance through different hormone-related responses.
摘要：
结论：生物刺激剂Hanseniassporaopuntiae调节拟南芥根的发育和对灰葡萄孢的抗性。有益的微生物可以增加植物营养的可及性和吸收，促进非生物胁迫耐受性，增强抗病性，而病原微生物引起植物病害,在最关键的情况下影响细胞稳态并导致细胞死亡。通常,植物使用专门的模式识别受体来感知有益或病原体微生物。尽管细菌是研究最多的植物相关有益微生物，酵母的分析受到的关注较少。这项研究评估了Hanseniassporaopuntiae的作用，从可可豆中分离出的发酵酵母，在拟南芥生长期间，发展,和对真菌病原体的防御反应。我们评估了A.thaliana-H.使用直接和间接体外系统的opuntiae相互作用。在间接相互作用期间，用H.opuntiae接种后7天，拟南芥的生长显着增加。此外，我们观察到H.opuntiae细胞在体外相互作用过程中在拟南芥根发育中具有很强的生长素样作用。我们表明，3-甲基-1-丁醇和乙醇是H.opuntiae产生的主要挥发性化合物。随后，确定接种了H.opuntiae的拟南芥植物对灰葡萄孢菌感染具有长期和系统性的作用，但独立于生长素，乙烯,水杨酸,或茉莉酸途径。我们的结果表明，H.opuntiae是一种重要的生物刺激剂，通过不同的激素相关反应调节植物发育和病原体抗性。
