PDF(Pubmed)
Abstract：
The polysaccharides found in Caulerpa lentillifera (sea grape algae) are potentially an important bioactive resource. This study makes use of RSM (response surface methodology) to determine the optimal conditions for the extraction of valuable SGP (sea grape polysaccharides). The findings indicated that a water/raw material ratio of 10:1 mL/g, temperature of 90 °C, and extraction time of 45 min would maximize the yield, with experimentation achieving a yield of 21.576 %. After undergoing purification through DEAE-52 cellulose and Sephacryl S-100 column chromatography, three distinct fractions were obtained, namely SGP11, SGP21, and SGP31, each possessing average molecular weights of 38.24 kDa, 30.13 kDa, and 30.65 kDa, respectively. Following characterization, the fractions were shown to comprise glucose, galacturonic acid, xylose, and mannose, while the sulfate content was in the range of 12.2-21.8 %. Using Fourier transform infrared spectroscopy (FT-IR) it was possible to confirm with absolute certainty the sulfate polysaccharide attributes of SGP11, SGP21, and SGP31. NMR (nuclear magnetic resonance) findings made it clear that SGP11 exhibited α-glycosidic configurations, while the configurations of SGP21 and SGP31 were instead β-glycosidic. The in vitro antioxidant assays which were conducted revealed that each of the fractions was able to demonstrate detectable scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and 2,2\'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cations. All fractions were also found to exhibit the capacity to scavenge NO radicals in a dose-dependent manner. SGP11, SGP21, and SGP31 were also able to display cellular antioxidant activity (CAA) against the human adenocarcinoma colon (Caco-2) cell line when oxidative damage was induced. The concentration levels were found to govern the extent of such activity. Moreover, purified SGP were found to exert strong inhibitory effects upon glycation, with the responses dependent upon dosage, thus confirming the potential for SGP to find a role as a natural resource for the production of polysaccharide-based antioxidant drugs, or products to promote improved health.
摘要：
在Caulerpalentillilifa（海葡萄藻类）中发现的多糖是潜在的重要生物活性资源。本研究利用RSM（响应面方法）来确定提取有价值的SGP（海葡萄多糖）的最佳条件。结果表明，水/原料比为10:1mL/g，温度为90°C，和45分钟的提取时间将最大限度地提高产量，通过实验获得21.576%的产率。经过DEAE-52纤维素和SephacrylS-100柱层析纯化后，获得了三个不同的馏分，即SGP11，SGP21和SGP31，每个具有38.24kDa的平均分子量，30.13kDa,30.65kDa，分别。在表征之后，这些部分被证明包含葡萄糖，半乳糖醛酸，木糖,和甘露糖,而硫酸盐含量在12.2-21.8%的范围内。使用傅立叶变换红外光谱（FT-IR），可以绝对确定地确认SGP11，SGP21和SGP31的硫酸盐多糖属性。NMR（核磁共振）的发现清楚地表明，SGP11表现出α-糖苷构型，而SGP21和SGP31的构型改为β-糖苷。进行的体外抗氧化剂测定表明，每个级分都能够证明对1,1-二苯基-2-吡啶酰肼（DPPH）自由基和2,2'-氮杂-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基阳离子的可检测清除活性。还发现所有级分都表现出以剂量依赖性方式清除NO自由基的能力。当诱导氧化损伤时,SGP11、SGP21和SGP31也能够显示针对人腺癌结肠(Caco-2)细胞系的细胞抗氧化活性(CAA)。发现浓度水平控制这种活性的程度。此外，发现纯化的SGP对糖基化有很强的抑制作用，反应取决于剂量，从而证实了SGP寻找作为生产基于多糖的抗氧化药物的自然资源的潜力，或促进健康改善的产品。
