PDF(Pubmed)
Abstract：
UNASSIGNED: In this study, it was aimed to investigate the possible effects of oral chewable probiotic tablets (PTs) produced to directly support the oral flora on the proliferation of human dental pulp stem cells (DPSCs) and human gingival fibroblast cells (HGFCs).
UNASSIGNED: For analysis in this study, \"Motiflor AS,\" a PT that dissolves in the mouth, containing 13.5mg Lactobacillus helveticus Rosell-52, L. rhamnosus Rosell-11, L. halivarus HA-118, and Bifidobacterium longum Rosell-175 was used. Cell survival and proliferation were analyzed by methyl-thiazole-diphenyl-tetrazolium (MTT) test and real-time cell analysis method (xCELLigence RTCA-DP) after 24-, 48-, and 72-h incubation periods.
UNASSIGNED: According to the data obtained with RTCA-DP software, there was a significant increase in the proliferation of human dental pulp stem cells (HDPSCs) and HGFCs in the 72-h incubation after PT application compared to the 24-h and 48-h incubations (P < 0.0001). After the MTT test, for HDPSCs, the cell proliferation rate was 62.8% and 85.6% in 24- and 48-h incubation, respectively, while HDPSCs cell proliferation rate in 72-h incubation was 135.2% (P < 0.0001). For HGFCs, the cell proliferation rate was 73% and 120.4% in 24- and 48-h incubation, respectively, while HDPSCs cell proliferation rate in 72-h incubation was 139.8% (P < 0.0001). When the results of the two tests applied were evaluated together, the results showed compatibility.
UNASSIGNED: Based on the results, it has been concluded that PT will be useful for maintaining oral health and for dental and gingival patients who will/have undergone dental treatment. It should be keep in mind that protecting our oral and dental health is very important in terms of protecting our general health.
UNASSIGNED: For analysis in this study, \"Motiflor AS,\" a PT that dissolves in the mouth, containing 13.5mg Lactobacillus helveticus Rosell-52, L. rhamnosus Rosell-11, L. halivarus HA-118, and Bifidobacterium longum Rosell-175 was used. Cell survival and proliferation were analyzed by methyl-thiazole-diphenyl-tetrazolium (MTT) test and real-time cell analysis method (xCELLigence RTCA-DP) after 24-, 48-, and 72-h incubation periods.
UNASSIGNED: According to the data obtained with RTCA-DP software, there was a significant increase in the proliferation of human dental pulp stem cells (HDPSCs) and HGFCs in the 72-h incubation after PT application compared to the 24-h and 48-h incubations (P < 0.0001). After the MTT test, for HDPSCs, the cell proliferation rate was 62.8% and 85.6% in 24- and 48-h incubation, respectively, while HDPSCs cell proliferation rate in 72-h incubation was 135.2% (P < 0.0001). For HGFCs, the cell proliferation rate was 73% and 120.4% in 24- and 48-h incubation, respectively, while HDPSCs cell proliferation rate in 72-h incubation was 139.8% (P < 0.0001). When the results of the two tests applied were evaluated together, the results showed compatibility.
UNASSIGNED: Based on the results, it has been concluded that PT will be useful for maintaining oral health and for dental and gingival patients who will/have undergone dental treatment. It should be keep in mind that protecting our oral and dental health is very important in terms of protecting our general health.
摘要：
■在这项研究中，旨在研究直接支持口腔菌群的口服咀嚼型益生菌片（PT）对人牙髓干细胞（DPSC）和人牙龈成纤维细胞（HGFCs）增殖的可能影响。
■对于本研究中的分析，\“MotiflorAS，“溶解在嘴里的PT，使用含有13.5mg瑞士乳杆菌Rosell-52、鼠李糖乳杆菌Rosell-11、halivarusL.HA-118和长双歧杆菌Rosell-175。24-后通过甲基-噻唑-二苯基-四唑（MTT）测试和实时细胞分析方法（xCELLigenceRTCA-DP）分析细胞存活和增殖48-，和72小时的潜伏期。
■根据RTCA-DP软件获得的数据，与24小时和48小时孵育相比，PT应用后72小时的人牙髓干细胞（HDPSC）和HGFCs的增殖显着增加（P<0.0001）。MTT试验后,对于HDPSC，培养24h和48h的细胞增殖率分别为62.8%和85.6%，分别,而HDPSCs在72h孵育中的细胞增殖率为135.2％（P<0.0001）。对于HGFCs，在24和48小时的孵育中，细胞增殖率分别为73%和120.4%，分别,而HDPSCs在72h孵育中的细胞增殖率为139.8%(P<0.0001)。当两个测试的结果一起评估时，结果显示了相容性。
■根据结果，已经得出结论，PT将有助于维持口腔健康以及将/已经接受牙科治疗的牙科和牙龈患者。应该记住，保护我们的口腔和牙齿健康对于保护我们的整体健康非常重要。
■对于本研究中的分析，\“MotiflorAS，“溶解在嘴里的PT，使用含有13.5mg瑞士乳杆菌Rosell-52、鼠李糖乳杆菌Rosell-11、halivarusL.HA-118和长双歧杆菌Rosell-175。24-后通过甲基-噻唑-二苯基-四唑（MTT）测试和实时细胞分析方法（xCELLigenceRTCA-DP）分析细胞存活和增殖48-，和72小时的潜伏期。
■根据RTCA-DP软件获得的数据，与24小时和48小时孵育相比，PT应用后72小时的人牙髓干细胞（HDPSC）和HGFCs的增殖显着增加（P<0.0001）。MTT试验后,对于HDPSC，培养24h和48h的细胞增殖率分别为62.8%和85.6%，分别,而HDPSCs在72h孵育中的细胞增殖率为135.2％（P<0.0001）。对于HGFCs，在24和48小时的孵育中，细胞增殖率分别为73%和120.4%，分别,而HDPSCs在72h孵育中的细胞增殖率为139.8%(P<0.0001)。当两个测试的结果一起评估时，结果显示了相容性。
■根据结果，已经得出结论，PT将有助于维持口腔健康以及将/已经接受牙科治疗的牙科和牙龈患者。应该记住，保护我们的口腔和牙齿健康对于保护我们的整体健康非常重要。
