UNASSIGNED: For analysis in this study, \"Motiflor AS,\" a PT that dissolves in the mouth, containing 13.5mg Lactobacillus helveticus Rosell-52, L. rhamnosus Rosell-11, L. halivarus HA-118, and Bifidobacterium longum Rosell-175 was used. Cell survival and proliferation were analyzed by methyl-thiazole-diphenyl-tetrazolium (MTT) test and real-time cell analysis method (xCELLigence RTCA-DP) after 24-, 48-, and 72-h incubation periods.
UNASSIGNED: According to the data obtained with RTCA-DP software, there was a significant increase in the proliferation of human dental pulp stem cells (HDPSCs) and HGFCs in the 72-h incubation after PT application compared to the 24-h and 48-h incubations (P < 0.0001). After the MTT test, for HDPSCs, the cell proliferation rate was 62.8% and 85.6% in 24- and 48-h incubation, respectively, while HDPSCs cell proliferation rate in 72-h incubation was 135.2% (P < 0.0001). For HGFCs, the cell proliferation rate was 73% and 120.4% in 24- and 48-h incubation, respectively, while HDPSCs cell proliferation rate in 72-h incubation was 139.8% (P < 0.0001). When the results of the two tests applied were evaluated together, the results showed compatibility.
UNASSIGNED: Based on the results, it has been concluded that PT will be useful for maintaining oral health and for dental and gingival patients who will/have undergone dental treatment. It should be keep in mind that protecting our oral and dental health is very important in terms of protecting our general health.
■对于本研究中的分析,\“MotiflorAS,“溶解在嘴里的PT,使用含有13.5mg瑞士乳杆菌Rosell-52、鼠李糖乳杆菌Rosell-11、halivarusL.HA-118和长双歧杆菌Rosell-175。24-后通过甲基-噻唑-二苯基-四唑(MTT)测试和实时细胞分析方法(xCELLigenceRTCA-DP)分析细胞存活和增殖48-,和72小时的潜伏期。
■根据RTCA-DP软件获得的数据,与24小时和48小时孵育相比,PT应用后72小时的人牙髓干细胞(HDPSC)和HGFCs的增殖显着增加(P<0.0001)。MTT试验后,对于HDPSC,培养24h和48h的细胞增殖率分别为62.8%和85.6%,分别,而HDPSCs在72h孵育中的细胞增殖率为135.2%(P<0.0001)。对于HGFCs,在24和48小时的孵育中,细胞增殖率分别为73%和120.4%,分别,而HDPSCs在72h孵育中的细胞增殖率为139.8%(P<0.0001)。当两个测试的结果一起评估时,结果显示了相容性。
■根据结果,已经得出结论,PT将有助于维持口腔健康以及将/已经接受牙科治疗的牙科和牙龈患者。应该记住,保护我们的口腔和牙齿健康对于保护我们的整体健康非常重要。