PDF(Pubmed)
Abstract：
The Coulomb potential maps generated by electron microscopy (EM) experiments contain not only information about the position but also about the charge state of the atom. This feature of EM maps allows the identification of specific ions and the protonation state of amino acid side chains in the sample. Here, we summarize qualitative observations of charges in EM maps, discuss the difficulties in interpreting the charge in Coulomb potential maps with respect to distinguishing it from radiation damage, and outline considerations to implement the correct charge in fitting algorithms.
摘要：
通过电子显微镜（EM）实验生成的库仑电势图不仅包含有关位置的信息，而且还包含有关原子电荷状态的信息。EM图谱的这一特征允许鉴定样品中的特定离子和氨基酸侧链的质子化状态。这里,我们总结了EM图中电荷的定性观察，讨论解释库仑电位图中电荷与辐射损伤的区别的困难，并概述了在拟合算法中实现正确收费的注意事项。
