OBJECTIVE: This study aims to assess the wound-healing potential of Salix aegyptiaca\'s flower ointment compared to phenytoin, considering the active constituents of S. aegyptiaca and its traditional usage.
METHODS: Initially, the active components of S. aegyptiaca were isolated and identified through the GC-MS technique. Subsequently, for the experimental intervention, thirty-five rats were divided into five distinct groups: control (C), phenytoin (F), and three S. aegyptiaca ointment groups at different concentrations (5 % - S5, 25 % - S25, and 50 % - S50). Anesthesia was administered, and wounds were induced on the animals\' necks following a standard procedure. These wounds were then treated for a duration of 21 days. Wound healing progress was quantified, and histopathological assessments were conducted using hematoxylin and eosin staining and Mason\'s trichrome staining.
RESULTS: The main active compounds of S. aegyptiaca, namely n-hexadecanoic acid and oleic acid, were identified via GC-MS analysis. Although the initial group weights did not show a significant difference (P = 0.271), a significant variation was observed in the final weights (P = 0.003). The S50 group exhibited a lower wound healing rate than the S25 group on the 7th and 14th days but surpassed it on the 21st day (C < F < S5≈S25
目的:本研究旨在评估与苯妥英钠相比,柳柳花软膏的伤口愈合潜力,考虑到埃及伊蚊的活性成分及其传统用法。
方法:最初,通过GC-MS技术分离并鉴定了埃及毛藻的活性成分。随后,对于实验性干预,将35只大鼠分为五个不同的组:对照组(C),苯妥英(F),和三个不同浓度的埃及酵母软膏组(5%-S5,25%-S25和50%-S50)。进行了麻醉,并按照标准程序在动物脖子上诱导伤口。然后将这些伤口处理21天的持续时间。量化伤口愈合进展,和组织病理学评估使用苏木精和伊红染色和梅森三色染色进行。
结果:埃及伊蚊的主要活性化合物,即正十六烷酸和油酸,通过GC-MS分析鉴定。尽管初始组的体重没有显着差异(P=0.271),在最终体重中观察到显著差异(P=0.003).S50组在第7天和第14天的伤口愈合率低于S25组,但在第21天超过了伤口愈合率(C