PDF(Pubmed)
Abstract：
Torque teno sus virus k2a (TTSuVk2a) is a member of the family Anelloviridae that can establish persistent infections in both domestic pigs and wild boars. Its association with diseases has not been precisely elucidated, and it is often considered only as a commensal virus. This infectious agent has been reported in herds throughout the world. In this study, we investigated the detection rate and diversity of TTSuVk2a in free-living wild boars from northeastern Patagonia, Argentina. Total DNA was extracted from tonsil samples of 50 animals, nested PCR assays were carried out, and infection was verified in 60% of the cases. Sequence analysis of the viral non-coding region revealed distinct phylogenetic groups. These clusters showed contrasting patterns of spatial distribution, which presented statistically significant differences when evaluating spatial aggregation. In turn, the sequences were compared with those available in the database to find that the clusters were distinguished by having similarity with TTSuVk2a variants of different geographic origin. The results suggested that Patagonian wild boar populations are bearers of diverse viral strains of Asian, European, and South American provenance.
摘要：
Torquetenosus病毒k2a（TTSuVk2a）是Anelloviridae家族的成员，可以在家猪和野猪中建立持续的感染。它与疾病的联系还没有被精确地阐明，它通常只被认为是共生病毒。这种传染性病原体已在世界各地的牧群中报道。在这项研究中,我们调查了来自巴塔哥尼亚东北部的自由生活野猪中TTSuVk2a的检出率和多样性，阿根廷。从50只动物的扁桃体样本中提取总DNA，进行巢式PCR检测，60%的病例证实感染。病毒非编码区的序列分析揭示了不同的系统发育群体。这些集群显示出不同的空间分布模式，在评估空间聚集时，这在统计上表现出显著的差异。反过来,将序列与数据库中可用的序列进行比较,发现通过与不同地理来源的TTSuVk2a变体具有相似性来区分簇.结果表明，巴塔哥尼亚野猪种群是亚洲不同病毒株的携带者，欧洲,和南美的出处。
