Abstract：
Studies in cows have reported that ovulation, steroidogenesis and angiogenesis are affected by stress and consequently fertility decreases. The purpose of this study was to evaluate the effects of ACTH administration during the preovulatory period on the expression of growth factors (CD-31, PDGF-A, PDGF-B, VEGFA-164, VEGFA-164b, VEGF-R1 and VEGF-R2) associated with the angiogenic process by immunohistochemistry in cows (n = 14). Results evidenced the expression of these growth factors in theca and granulosa cells from antral, atretic and dominant preovulatory follicles of ACTH-treated cows, suggesting that, under stress conditions, their expression continues to be required. VEGFA-164, VEGF-R1 and VEGF-R2 expression was greater in theca cells of dominant preovulatory follicles of the ACTH-treated group than in those of the control group. CD-31 protein expression was lower in the dominant preovulatory follicles of the ACTH-treated group than in those of the control group. PDGF-A and PDGF-B expression did not differ between groups, either in granulosa or in theca cells. These results suggest that VEGFA-164, its receptors and CD-31 are actors in the normal cycle of the ovaries and could have greater pathophysiological importance in the altered angiogenic process and other events that occur during anovulation and stress conditions. This dysregulation reinforces the importance of the angiogenic process in the pathophysiology of cystic ovarian disease in cows. This is the first report on the expression and localization of components of the VEGF and PDGF systems and CD-31 in cells from dominant preovulatory follicles after ACTH administration.
摘要：
对奶牛的研究报告说，排卵，类固醇生成和血管生成受到压力的影响，因此生育能力下降。本研究的目的是评估排卵前期ACTH给药对生长因子表达的影响（CD-31，PDGF-A，PDGF-B,VEGFA-164,VEGFA-164b,VEGF-R1和VEGF-R2）通过免疫组织化学与奶牛的血管生成过程相关（n=14）。结果表明，这些生长因子在卵泡膜和颗粒细胞中的表达来自窦，ACTH处理母牛的闭锁和优势排卵前卵泡，这表明，在应力条件下，他们的表达仍然是必需的。ACTH治疗组的优势排卵前卵泡膜细胞中的VEGFA-164，VEGF-R1和VEGF-R2表达高于对照组。ACTH治疗组的优势排卵前卵泡中CD-31蛋白表达低于对照组。PDGF-A和PDGF-B的表达在组间没有差异，在颗粒细胞或卵泡膜细胞中。这些结果表明，VEGFA-164，其受体和CD-31是卵巢正常周期的参与者，并且在血管生成过程和无排卵和应激条件下发生的其他事件中可能具有更大的病理生理学重要性。这种失调加强了血管生成过程在奶牛囊性卵巢疾病的病理生理学中的重要性。这是关于在ACTH施用后来自优势排卵前卵泡的细胞中VEGF和PDGF系统和CD-31的组分的表达和定位的首次报道。
