Abstract：
Temporary immersion systems (TIS) have been used for orchid micropropagation. The main advantage of TIS use for micropropagation is that the explant is periodically immersed in nutrient media, and then, the nutrient solution is drained, which allows the explant tissue to stay in air. The current review resumes the application of TIS in orchid propagation. Fifty-three papers are discussed considering: explant, culture media, TIS bioreactor type, frequency and immersion time, and the TIS effects in acclimatization phase.
摘要：
临时浸没系统（TIS）已用于兰花微繁殖。TIS用于微繁殖的主要优点是外植体定期浸入营养培养基中，然后,营养液排干,这允许外植体组织留在空气中。本文综述了TIS在兰花繁殖中的应用。讨论了53篇论文，考虑到：外植体，文化媒介,TIS生物反应器类型,频率和浸泡时间，以及适应阶段的TIS效应。
