PDF(Pubmed)
Abstract：
Primary cilia are microtubule-based sensory organelles that project from the apical surface of most mammalian cells, including oligodendrocytes, which are myelinating cells of the central nervous system (CNS) that support critical axonal function. Dysfunction of CNS glia is associated with aging-related white matter diseases and neurodegeneration, and ciliopathies are known to affect CNS white matter. To investigate age-related changes in ciliary profile, we examined ciliary length and frequency in the retinogeniculate pathway, a white matter tract commonly affected by diseases of aging but in which expression of cilia has not been characterized. We found expression of Arl13b, a marker of primary cilia, in a small group of Olig2-positive oligodendrocytes in the optic nerve, optic chiasm, and optic tract in young and aged C57BL/6 wild-type mice. While the ciliary length and ciliated oligodendrocyte cells were constant in young mice in the retinogeniculate pathway, there was a significant increase in ciliary length in the anterior optic nerve as compared to the aged animals. Morphometric analysis confirmed a specific increase in the ciliation rate of CC1+ /Olig2+ oligodendrocytes in aged mice compared with young mice. Thus, the prevalence of primary cilia in oligodendrocytes in the visual pathway and the age-related changes in ciliation suggest that they may play important roles in white matter and age-associated optic neuropathies.
摘要：
初级纤毛是基于微管的感觉细胞器，从大多数哺乳动物细胞的顶端表面突出，包括少突胶质细胞,它们是中枢神经系统（CNS）的髓鞘细胞，支持关键的轴突功能。中枢神经系统神经胶质的功能障碍与衰老相关的白质疾病和神经变性有关，已知纤毛病变会影响中枢神经系统白质。为了调查纤毛轮廓的年龄相关变化，我们检查了视黄酶通路的纤毛长度和频率，通常受衰老疾病影响的白质道，但其中纤毛的表达尚未得到表征。我们发现Arl13b的表达,初级纤毛的标记,在视神经中的一小组Olig2阳性少突胶质细胞中，视神经交叉，年轻和老年C57BL/6野生型小鼠的视神经束。虽然幼鼠的纤毛长度和纤毛少突胶质细胞在视黄酶途径中是恒定的，与老年动物相比，前视神经的睫状长度显着增加。形态计量学分析证实，与年轻小鼠相比，老年小鼠中CC1/Olig2少突胶质细胞的分裂率有特定的增加。因此，视通路少突胶质细胞中原发性纤毛的患病率和与年龄相关的纤毛变化提示,它们可能在白质和与年龄相关的视神经病变中发挥重要作用.
