PDF(Pubmed)
Abstract：
UNASSIGNED: This study aimed to determine the sex and estimate the age of individuals by analysing Raman spectra obtained from extracted teeth dentine.
UNASSIGNED: A total of 25 male and 26 female extracted teeth were collected, disinfected, and fixed in auto acrylate before being cut near the cervical margin. The inclusion criteria were non endodontically treated teeth with the cervical third of the tooth crown preserved. The exposed dentine was recorded using a Raman spectrometer, with 100 scans per dentine site in the spectral range of 3500 to 200 cm-1 and a resolution of 4 cm-1. Background-corrected, normalized, and decomposed spectra were subjected to principal component analysis using MATLAB 2010 (The MathWorks, Natick, MA, USA). To assess age differences, prominent vibrational bands were observed and statistically analyzed, while sex differentiation employed the T-test on acquired data, exploring vibrational bands with significant intensity differences.
UNASSIGNED: The obtained results revealed a segmentation of spectra in the male sample group based on the age of teeth at extraction, with an accuracy of age estimation at 7.0048 years. A similar segmentation was observed in the female sample group, with an accuracy of 9.3863 years.
UNASSIGNED: The recorded spectra were organized into datasets and analyzed with principal component analysis, demonstrating the feasibility of sex determination and age estimation using Raman spectra of teeth. Differences in classification accuracy between sexes may be attributed to hormonally-mediated differences in the biochemical composition of dentine between males and females. The findings of this study can provide valuable insights into methods for forensic and anthropological applications.
UNASSIGNED: A total of 25 male and 26 female extracted teeth were collected, disinfected, and fixed in auto acrylate before being cut near the cervical margin. The inclusion criteria were non endodontically treated teeth with the cervical third of the tooth crown preserved. The exposed dentine was recorded using a Raman spectrometer, with 100 scans per dentine site in the spectral range of 3500 to 200 cm-1 and a resolution of 4 cm-1. Background-corrected, normalized, and decomposed spectra were subjected to principal component analysis using MATLAB 2010 (The MathWorks, Natick, MA, USA). To assess age differences, prominent vibrational bands were observed and statistically analyzed, while sex differentiation employed the T-test on acquired data, exploring vibrational bands with significant intensity differences.
UNASSIGNED: The obtained results revealed a segmentation of spectra in the male sample group based on the age of teeth at extraction, with an accuracy of age estimation at 7.0048 years. A similar segmentation was observed in the female sample group, with an accuracy of 9.3863 years.
UNASSIGNED: The recorded spectra were organized into datasets and analyzed with principal component analysis, demonstrating the feasibility of sex determination and age estimation using Raman spectra of teeth. Differences in classification accuracy between sexes may be attributed to hormonally-mediated differences in the biochemical composition of dentine between males and females. The findings of this study can provide valuable insights into methods for forensic and anthropological applications.
摘要：
■本研究旨在通过分析从提取的牙齿牙本质获得的拉曼光谱来确定个体的性别和年龄。
■共收集了25颗男性和26颗女性拔牙，消毒,并固定在自动丙烯酸酯中，然后在宫颈边缘附近切开。纳入标准是非牙髓治疗的牙齿，保留了牙冠的颈部三分之一。使用拉曼光谱仪记录暴露的牙本质，每个牙本质部位扫描100次，光谱范围为3500至200cm-1，分辨率为4cm-1。背景更正，归一化，并使用MATLAB2010对分解后的光谱进行主成分分析(MathWorks，纳蒂克,MA,美国）。为了评估年龄差异，观察并统计分析突出的振动带，虽然性别分化对获得的数据采用T检验，探索具有显著强度差异的振动带。
■获得的结果表明，根据拔牙时的牙齿年龄，对男性样本组中的光谱进行了分割，年龄估计的准确性为7.0048岁。在女性样本组中观察到类似的分割，精度为9.3863年。
■将记录的光谱组织成数据集，并通过主成分分析进行分析，证明使用牙齿的拉曼光谱确定性别和年龄估计的可行性。性别之间分类准确性的差异可能归因于激素介导的男性和女性之间牙本质生化组成的差异。这项研究的结果可以为法医和人类学应用方法提供有价值的见解。
■共收集了25颗男性和26颗女性拔牙，消毒,并固定在自动丙烯酸酯中，然后在宫颈边缘附近切开。纳入标准是非牙髓治疗的牙齿，保留了牙冠的颈部三分之一。使用拉曼光谱仪记录暴露的牙本质，每个牙本质部位扫描100次，光谱范围为3500至200cm-1，分辨率为4cm-1。背景更正，归一化，并使用MATLAB2010对分解后的光谱进行主成分分析(MathWorks，纳蒂克,MA,美国）。为了评估年龄差异，观察并统计分析突出的振动带，虽然性别分化对获得的数据采用T检验，探索具有显著强度差异的振动带。
■获得的结果表明，根据拔牙时的牙齿年龄，对男性样本组中的光谱进行了分割，年龄估计的准确性为7.0048岁。在女性样本组中观察到类似的分割，精度为9.3863年。
■将记录的光谱组织成数据集，并通过主成分分析进行分析，证明使用牙齿的拉曼光谱确定性别和年龄估计的可行性。性别之间分类准确性的差异可能归因于激素介导的男性和女性之间牙本质生化组成的差异。这项研究的结果可以为法医和人类学应用方法提供有价值的见解。
