Abstract：
Hydrogen peroxide is widely used to remedy bacterial and parasitic infections, but its excessive use will cause severe damage to aquatic animals. Moreover, there is no safe, efficient and low-cost method to degrade residual hydrogen peroxide in water. Here we developed a hydrogen peroxide removal mechanism by which autoinducer-2 (AI-2), a quorum sensing signal molecule that can promote the hydrogen peroxide degradation by Gram-positive bacteria. Here, we investigated the promotion effect of AI-2 on hydrogen peroxide degradation by Deinococcus sp. Y35 and the response of the antioxidant system. We further sought to understand the key mechanism underlying the promotion effect of AI-2 on hydrogen peroxide degradation is that, AI-2 contributed to the resistance of strain Y35 to oxidative stress induced by hydrogen peroxide, and altered membrane permeability of strain Y35 that allowed more hydrogen peroxide to enter bacterial cells and be degraded. Additionally, AI-2 can also encourage multiple Gram-positive bacteria to degrade hydrogen peroxide. Accordingly, our study serves as a reference for the regulation mechanism of the signal molecule AI-2 and provides the development of new strategies for hydrogen peroxide degradation.
摘要：
过氧化氢广泛用于治疗细菌和寄生虫感染，但是过度使用会对水生动物造成严重损害。此外，没有安全,高效、低成本的降解水中残留过氧化氢的方法。在这里，我们开发了一种过氧化氢去除机制，自动诱导物-2(AI-2)，一种群体感应信号分子，可以促进革兰氏阳性细菌对过氧化氢的降解。这里,我们研究了AI-2对异常球菌降解过氧化氢的促进作用。Y35和响应的抗氧化体系。我们进一步试图了解AI-2对过氧化氢降解的促进作用的关键机制是，AI-2有助于菌株Y35对过氧化氢诱导的氧化应激的抗性，和改变菌株Y35的膜通透性，使更多的过氧化氢进入细菌细胞并被降解。此外,AI-2还可以鼓励多种革兰氏阳性菌降解过氧化氢。因此，我们的研究为信号分子AI-2的调节机制提供了参考，并为过氧化氢降解提供了新的策略。
