Abstract：
Hypopharyngeal squamous cell carcinoma (HPSCC) has the worst prognosis among head and neck squamous cell carcinomas. The lack of available tumor cell lines poses a significant obstacle to the development of efficient treatments for HPSCC. In this study, we successfully established a novel cell line, named CZH1, from the postcricoid region of a Chinese male patient with a T3N0M0 HPSCC. Short tandem repeat analysis confirmed the uniqueness of CZH1. The cell line was characterized by its phenotypes, biomarkers, and genetics. Importantly, CZH1 cells retained the typical features of epithelial malignancy, similar to the primary tumor tissue. Furthermore, CZH1 demonstrated a greater capacity for invasion and increased susceptibility to irradiation in comparison to FaDu, which is the most commonly used HPSCC cell line. Whole-exome sequencing analysis revealed that CZH1 cells had typical genomic features of HNSCC, including mutations of TP53 and amplifications of multiple transcripts. Therefore, our newly developed CZH1 cell line could serve as an efficient tool for the in vitro investigation of the etiology, pathogenesis, and preclinical treatment of HPSCC.
摘要：
下咽鳞状细胞癌（HPSCC）在头颈部鳞状细胞癌中预后最差。缺乏可用的肿瘤细胞系对开发有效的HPSCC治疗构成了重大障碍。在这项研究中,我们成功地建立了一个新的细胞系,命名为CZH1，来自中国男性T3N0M0HPSCC患者的环状突后区域。短串联重复分析证实了CZH1的独特性。该细胞系的特征在于其表型，生物标志物，和遗传学。重要的是,CZH1细胞保留了上皮恶性肿瘤的典型特征，与原发肿瘤组织相似。此外，与FaDu相比，CZH1表现出更大的侵袭能力和更高的辐照敏感性，是最常用的HPSCC细胞系。全外显子组测序分析显示CZH1细胞具有典型的HNSCC基因组特征,包括TP53的突变和多个转录物的扩增。因此,我们新开发的CZH1细胞系可以作为体外研究病因的有效工具，发病机制，和HPSCC的临床前治疗。
