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Abstract：
The objective was to investigate the embryo morphokinitics using a time-lapse monitoring (TLM) system and assessment of clinical outcomes following intracytoplasmic sperm injection (ICSI) with zona pellucida (ZP)-bound sperm selection and conventional methods. A total of 371 metaphase II (MII) oocytes from 50 ICSI cycles were studied. Sibling oocytes were randomly divided into control (n = 199) and ZP-bound group (n = 172). All resulting zygotes were cultured and monitored in the TLM system up to Day 3 after ICSI. Fertilization rate, early embryo development, and clinical outcomes were evaluated. No significant differences were found in fertilization rate, time-lapse qualitative and quantitative measures, pronuclear fading time (PNF) t2, t3, t4, t5, t6, and t7 (times of cleavage to 2, 3, 4, 5, 6, and 7 cells), respectively. However, the t8 (time of cleavage to eight cells) and cc3 (duration of third cell cycle) revealed a significant difference between control and ZP-bound groups (p < .05). A significant difference between the two groups (p < .05) in the rates of Grade A embryos (according to Basile algorithm), chemical pregnancy, clinical pregnancy, and implantation was observed. Sperm selection using biological materials, such as ZP, improved both embryo quality and pregnancy outcomes, despite not affecting the early embryo development and morphokinetic parameters up to t8. This prospective randomized sibling oocyte trial was registered in October 2020 to January 2022 (IRCT20200705048021N1).
摘要：
目的是使用延时监测（TLM）系统研究胚胎形态动力学，并评估卵胞浆内单精子注射（ICSI）结合透明带（ZP）结合精子选择和常规方法的临床结果。研究了来自50个ICSI周期的371个中期II（MII）卵母细胞。将同胞卵母细胞随机分为对照组（n=199）和ZP结合组（n=172）。在ICSI后第3天,在TLM系统中培养并监测所有得到的受精卵。施肥率,早期胚胎发育,并对临床结局进行评价.受精率差异不显著,延时定性和定量措施，原核褪色时间（PNF）t2，t3，t4，t5，t6和t7（分裂到2，3，4，5，6和7细胞的时间），分别。然而,t8（裂解8个细胞的时间）和cc3（第三个细胞周期的持续时间）显示对照组和ZP结合组之间存在显着差异（p<0.05）。两组的A级胚胎率（根据Basile算法）之间存在显着差异（p<0.05），化学妊娠,临床妊娠,并观察到植入。使用生物材料选择精子，比如ZP,改善胚胎质量和妊娠结局，尽管不影响早期胚胎发育和形态动力学参数，直到t8。这项前瞻性随机同胞卵母细胞试验于2020年10月至2022年1月注册（IRCT20200705048021N1）。
