PDF(Pubmed)
Abstract：
Sepsis involves an immunological systemic response to a microbial pathogenic insult, leading to a cascade of interconnected biochemical, cellular, and organ-organ interaction networks. Potential drug targets can depict aquaporins, as they are involved in immunological processes. In immune cells, AQP3 and AQP9 are of special interest. In this study, we tested the hypothesis that these aquaporins are expressed in the blood cells of septic patients and impact sepsis survival. Clinical data, routine laboratory parameters, and blood samples from septic patients were analyzed on day 1 and day 8 after sepsis diagnosis. AQP expression and cytokine serum concentrations were measured. AQP3 mRNA expression increased over the duration of sepsis and was correlated with lymphocyte count. High AQP3 expression was associated with increased survival. In contrast, AQP9 expression was not altered during sepsis and was correlated with neutrophil count, and low levels of AQP9 were associated with increased survival. Furthermore, AQP9 expression was an independent risk factor for sepsis lethality. In conclusion, AQP3 and AQP9 may play contrary roles in the pathophysiology of sepsis, and these results suggest that AQP9 may be a novel drug target in sepsis and, concurrently, a valuable biomarker of the disease.
摘要：
脓毒症涉及对微生物病原损伤的免疫系统反应，导致一系列相互关联的生化，细胞，和器官-器官相互作用网络。潜在的药物靶标可以描述水通道蛋白，因为它们参与免疫过程。在免疫细胞中，AQP3和AQP9是特别感兴趣的。在这项研究中,我们检验了以下假设:这些水通道蛋白在脓毒症患者的血细胞中表达并影响脓毒症患者的生存.临床数据,常规实验室参数,在脓毒症诊断后第1天和第8天分析脓毒症患者的血液样本。测量AQP表达和细胞因子血清浓度。AQP3mRNA表达在脓毒症持续时间内增加，并与淋巴细胞计数相关。AQP3高表达与生存率增加有关。相比之下,AQP9表达在脓毒症期间没有改变，并且与中性粒细胞计数相关，低水平的AQP9与生存率增加有关。此外，AQP9表达是脓毒症致死率的独立危险因素。总之,AQP3和AQP9在脓毒症的病理生理过程中可能发挥相反的作用。这些结果表明AQP9可能是脓毒症的一个新的药物靶点,同时,这种疾病的有价值的生物标志物。
