Abstract：
As an effective cell assembly method, three-dimensional bioprinting has been widely used in building organ models and tissue repair over the past decade. However, different shear stresses induced throughout the entire printing process can cause complex impacts on cell integrity, including reducing cell viability, provoking morphological changes and altering cellular functionalities. The potential effects that may occur and the conditions under which these effects manifest are not clearly understood. Here, we review systematically how different mammalian cells respond under shear stress. We enumerate available experimental apparatus, and we categorise properties that can be affected under disparate stress patterns. We also summarise cell damaging mathematical models as a predicting reference for the design of bioprinting systems. We concluded that it is essential to quantify specific cell resistance to shear stress for the optimisation of bioprinting systems. Besides, as substantial positive impacts, including inducing cell alignment and promoting cell motility, can be generated by shear stress, we suggest that we find the proper range of shear stress and actively utilise its positive influences in the development of future systems.
摘要：
作为一种有效的电池组装方法，在过去的十年中，3D生物打印已广泛用于构建器官模型和组织修复。然而,在整个打印过程中引起的不同剪切应力会对电池完整性造成复杂的影响，包括降低细胞活力，引起形态学变化和改变细胞功能。可能发生的潜在影响以及这些影响显现的条件尚不清楚。这里,我们系统地回顾了不同哺乳动物细胞在剪切应力下的反应。我们列举了可用的实验仪器，我们对不同压力模式下可能受到影响的属性进行分类。我们还总结了细胞损伤数学模型，作为生物打印系统设计的预测参考。我们得出的结论是，量化特定细胞对剪切应力的抵抗力对于优化生物打印系统至关重要。此外,作为重大的积极影响，包括诱导细胞排列和促进细胞运动，可以通过剪应力产生，我们建议我们找到合适的剪应力范围，并积极利用其在未来系统开发中的积极影响。
