PDF(Pubmed)
Abstract：
Campylobacter concisus is an oral bacterium. Recent studies suggest that C. concisus may be involved in human gastric diseases. The mechanisms, however, by which C. concisus causes human gastric diseases have not been investigated. Here we examined the gastric epithelial pathogenicity of C. concisus using a cell culture model. Six C. concisus strains and the human gastric epithelial cell line AGS cells were used. IL-8 produced by AGS cells after incubation with C. concisus was measured using enzyme-linked immunosorbent assay (ELISA), and AGS cell apoptosis was determined by caspase 3/7 activities. The effects of C. concisus on actin arrangement in AGS cells was determined using fluorescence staining. The effects of C. concisus on global gene expression in AGS cells was determined by transcriptomic analysis and quantitative real-time PCR (qRT-PCR). The role of the upregulated CYP1A1 gene in gastric cancer survival was assessed using the Kaplan-Meier method. C. concisus induced production of IL-8 by AGS cells with strain variation. Significantly increased caspase 3/7 activities were observed in AGS cells incubated with C. concisus strains when compared to AGS cells without bacteria. C. concisus induced actin re-arrangement in AGS cells. C. concisus upregulated 30 genes in AGS cells and the upregulation of CYP1A1 gene was confirmed by qRT-PCR. The Kaplan-Meier analysis showed that upregulation of CYP1A1 gene is associated with worse survival in gastric cancer patients. Our findings suggest that C. concisus may play a role in gastric inflammation and the progression of gastric cancer. Further investigation in clinical studies is warranted.
摘要：
弯曲杆菌是一种口腔细菌。最近的研究表明C.concisus可能与人类胃病有关。机制，然而,C.concisus引起人类胃部疾病的原因尚未得到研究。在这里，我们使用细胞培养模型检查了C.concisus的胃上皮致病性。使用6种C.concisus菌株和人胃上皮细胞系AGS细胞。用酶联免疫吸附试验(ELISA)测定AGS细胞在与菊苣孵育后产生的IL-8,通过caspase3/7活性测定AGS细胞凋亡。使用荧光染色确定了C.concisus对AGS细胞中肌动蛋白排列的影响。通过转录组学分析和定量实时PCR(qRT-PCR)确定了菊花对AGS细胞中整体基因表达的影响。使用Kaplan-Meier方法评估了上调的CYP1A1基因在胃癌存活中的作用。C.concisus诱导AGS细胞产生IL-8，具有菌株变异。当与没有细菌的AGS细胞相比时,在用C.concisus菌株孵育的AGS细胞中观察到显著增加的半胱天冬酶3/7活性。C.concisus诱导AGS细胞中的肌动蛋白重排。C.concisus在AGS细胞中上调了30种基因，并通过qRT-PCR证实了CYP1A1基因的上调。Kaplan-Meier分析显示，CYP1A1基因上调与胃癌患者的生存率降低相关。我们的发现表明C.concisus可能在胃部炎症和胃癌的进展中起作用。在临床研究中需要进一步的研究。
