PDF(Pubmed)
Abstract：
Neoplastic cells need to adapt their gene expression pattern to survive in an ever-changing or unfavorable tumor microenvironment. Protein synthesis (or mRNA translation), an essential part of gene expression, is dysregulated in cancer. The emergence of distinct translatomic technologies has revolutionized oncological studies to elucidate translational regulatory mechanisms. Ribosome profiling can provide adequate information on diverse aspects of translation by aiding in quantitatively analyzing the intensity of translating ribosome-protected fragments. Here, we review the primary currently used translatomics techniques and highlight their advantages and disadvantages as tools for translatomics studies. Subsequently, we clarified the areas in which ribosome profiling could be applied to better understand translational control. Finally, we summarized the latest advances in cancer studies using ribosome profiling to highlight the extensive application of this powerful and promising translatomic tool.
摘要：
肿瘤细胞需要适应其基因表达模式才能在不断变化或不利的肿瘤微环境中生存。蛋白质合成（或mRNA翻译），基因表达的重要组成部分，在癌症中失调。独特的翻译技术的出现彻底改变了肿瘤学研究，以阐明翻译调节机制。核糖体谱分析可以通过帮助定量分析翻译核糖体保护片段的强度来提供有关翻译各个方面的足够信息。这里,我们回顾了目前主要使用的翻译组学技术，并强调了它们作为翻译组学研究工具的优缺点。随后，我们阐明了核糖体谱分析可用于更好地理解翻译控制的领域.最后,我们总结了使用核糖体谱分析的癌症研究的最新进展，以强调这种强大而有前途的翻译工具的广泛应用。
