Abstract：
The Ames MPF™ is a miniaturized, microplate fluctuation format of the Ames test. It is a standardized, commercially available product which can be used to assess mutagenicity in Salmonella and E. coli strains in 384-well plates using a color change-based readout. Several peer-reviewed comparisons of the Ames MPF™ to the Ames test in Petri dishes confirmed its suitability to evaluate the mutagenic potential of a variety of test items. An international multicenter study involving seven laboratories tested six coded chemicals with this assay using five bacterial strains, as recommended by the OECD test guideline 471. The data generated by the participating laboratories was in excellent agreement (93%), and the similarity of their dose response curves, as analyzed with sophisticated statistical approaches further confirmed the suitability of the Ames MPF™ assay as an alternative to the Ames test on agar plates, but with advantages with respect to significantly reduced amount of test substance and S9 requirements, speed, hands-on time and, potentially automation.
摘要：
AmesMPF™是一款小型化的，Ames试验的微孔板波动格式。这是一个标准化的,可用于使用基于颜色变化的读数在384孔板中评估沙门氏菌和大肠杆菌菌株的诱变性的市售产品。AmesMPF™与培养皿中Ames测试的若干同行评审比较证实了其适合于评估各种测试项目的诱变潜力。一项涉及七个实验室的国际多中心研究使用五种细菌菌株测试了六种编码化学物质，按照经合组织测试准则471的建议。参与实验室产生的数据非常一致(93%)，以及它们的剂量反应曲线的相似性，通过复杂的统计方法分析，进一步证实了AmesMPF™测定作为琼脂平板上Ames测试的替代方法的适用性，但在显著减少受试物质的量和S9要求方面具有优势，速度,动手时间和,潜在的自动化。
