Abstract：
The current study was designed to test a functional food (FF) mixture containing aldose reductase inhibitors and antiglycation bioactive compounds for suppressing the onset and progression of cataracts in a diabetic rat model. Two-month-old Sprague Dawley rats were grouped as control (C), diabetes untreated (D), and diabetic rats treated with FF at two doses (FF1 = 1.35 g and FF2 = 6.25 g/100g of diet). Diabetes was induced by a single injection of streptozotocin. The FF is a mixture of amla, turmeric, black pepper, cinnamon, ginger, and fenugreek added to the rodent diet. The status of cataracts was monitored weekly by a slit lamp examination for 20 weeks, after which animals were sacrificed to collect eye lenses. Feeding FF1 and FF2 to diabetic rats yielded a significant anti-hyperglycaemic effect and marginally prevented body weight loss. FF delayed cataract progression, and FF2 showed better efficacy than FF1. FF prevented the loss of lens crystallins and their insolubilization in diabetic rats. The antioxidant potential of FF was evident with the lowered protein carbonyls, lipid peroxidation, and prevention of altered antioxidant enzyme activities induced by diabetes. These studies demonstrate the efficacy of plant-derived dietary supplements against the onset and progression of cataracts in a well-established rat model of diabetic eye disease.
摘要：
本研究旨在测试含有醛糖还原酶抑制剂和抗糖基化生物活性化合物的功能性食品（FF）混合物，以抑制糖尿病大鼠模型中白内障的发作和进展。将两个月大的SpragueDawley大鼠分组为对照组（C），未经治疗的糖尿病（D），和用两种剂量的FF处理的糖尿病大鼠(FF1=1.35g和FF2=6.25g/100g饮食)。通过单次注射链脲佐菌素诱发糖尿病。FF是amla的混合物，姜黄,黑胡椒,肉桂,Ginger,和胡芦巴添加到啮齿动物的饮食。通过裂隙灯检查每周监测白内障的状况，持续20周，之后处死动物以收集眼晶状体。向糖尿病大鼠喂食FF1和FF2可产生显着的抗高血糖作用，并略微防止体重减轻。FF延迟白内障进展，FF2的疗效优于FF1。FF可防止糖尿病大鼠晶状体晶体蛋白的丢失及其不溶解。FF的抗氧化潜力与降低的蛋白质羰基是明显的，脂质过氧化，和预防糖尿病引起的抗氧化酶活性改变。这些研究证明了植物源性膳食补充剂在已建立的糖尿病眼病大鼠模型中对白内障发作和进展的功效。
