PDF(Pubmed)
Abstract：
BACKGROUND: The immunomodulatory properties of human chorionic gonadotrophin (hCG) have been identified to be critical for successful pregnancy. However, the effects of hCG on peripheral γδT cells during early pregnancy have not been reported previously.
METHODS: We cocultured the purified γδT cells and peripheral blood mononuclear cells (PBMCs) with early pregnancy-relevant hCG concentrations and investigated the changes in the immune functional characteristics of γδT cells via flow cytometry assays.
RESULTS: The ratios of CD69+ and IL-10+ γδT cells were increased in early pregnant women compared to nonpregnant women. γδT cells expressed low levels of the mannose receptor (CD206) instead of the classical hCG/LH receptor for hCG. The direct treatment of purified γδT cells with early pregnancy-relevant hCG concentrations may have no significant effects on their immune functions. Interestingly, when PBMCs were treated with the same broad range of hCG concentrations, the ratios of CD69+ and IL-10+ γδT cells to total γδT cells were significantly increased.
CONCLUSIONS: Certain early pregnancy-relevant hCG concentrations could enhance the ratios of peripheral CD69+ and IL-10+ γδT cells, contributing to the activation of γδT cells and immunological tolerance during early pregnancy. However, these affects may not be strongly mediated by direct ligand-receptor interactions and they may highly depend on immune microenvironment. Our novel observations propose a perspective into the endocrine-immune dialog that exists between the fetus and maternal immune cells.
METHODS: We cocultured the purified γδT cells and peripheral blood mononuclear cells (PBMCs) with early pregnancy-relevant hCG concentrations and investigated the changes in the immune functional characteristics of γδT cells via flow cytometry assays.
RESULTS: The ratios of CD69+ and IL-10+ γδT cells were increased in early pregnant women compared to nonpregnant women. γδT cells expressed low levels of the mannose receptor (CD206) instead of the classical hCG/LH receptor for hCG. The direct treatment of purified γδT cells with early pregnancy-relevant hCG concentrations may have no significant effects on their immune functions. Interestingly, when PBMCs were treated with the same broad range of hCG concentrations, the ratios of CD69+ and IL-10+ γδT cells to total γδT cells were significantly increased.
CONCLUSIONS: Certain early pregnancy-relevant hCG concentrations could enhance the ratios of peripheral CD69+ and IL-10+ γδT cells, contributing to the activation of γδT cells and immunological tolerance during early pregnancy. However, these affects may not be strongly mediated by direct ligand-receptor interactions and they may highly depend on immune microenvironment. Our novel observations propose a perspective into the endocrine-immune dialog that exists between the fetus and maternal immune cells.
摘要：
背景：已经确定人绒毛膜促性腺激素（hCG）的免疫调节特性对于成功怀孕至关重要。然而,hCG对妊娠早期外周血γδT细胞的影响尚未见报道。
方法：我们将纯化的γδT细胞和外周血单核细胞（PBMC）与早孕相关的hCG浓度共培养，并通过流式细胞术检测γδT细胞免疫功能特征的变化。
结果：与未怀孕妇女相比，妊娠早期妇女中CD69+和IL-10+γδT细胞的比例增加。γδT细胞表达低水平的甘露糖受体（CD206），而不是hCG的经典hCG/LH受体。用早期妊娠相关的hCG浓度直接处理纯化的γδT细胞可能对其免疫功能没有显着影响。有趣的是,当PBMC用相同范围的hCG浓度处理时，CD69和IL-10γδT细胞与总γδT细胞的比例显着增加。
结论：某些早孕相关的hCG浓度可以提高外周血CD69+和IL-10+γδT细胞的比例，促进妊娠早期γδT细胞的激活和免疫耐受。然而,这些影响可能不是由直接的配体-受体相互作用强烈介导的,它们可能高度依赖于免疫微环境.我们的新观察为胎儿和母体免疫细胞之间存在的内分泌-免疫对话提供了一个视角。
方法：我们将纯化的γδT细胞和外周血单核细胞（PBMC）与早孕相关的hCG浓度共培养，并通过流式细胞术检测γδT细胞免疫功能特征的变化。
结果：与未怀孕妇女相比，妊娠早期妇女中CD69+和IL-10+γδT细胞的比例增加。γδT细胞表达低水平的甘露糖受体（CD206），而不是hCG的经典hCG/LH受体。用早期妊娠相关的hCG浓度直接处理纯化的γδT细胞可能对其免疫功能没有显着影响。有趣的是,当PBMC用相同范围的hCG浓度处理时，CD69和IL-10γδT细胞与总γδT细胞的比例显着增加。
结论：某些早孕相关的hCG浓度可以提高外周血CD69+和IL-10+γδT细胞的比例，促进妊娠早期γδT细胞的激活和免疫耐受。然而,这些影响可能不是由直接的配体-受体相互作用强烈介导的,它们可能高度依赖于免疫微环境.我们的新观察为胎儿和母体免疫细胞之间存在的内分泌-免疫对话提供了一个视角。
