关键词: 25(OH)D Diet ELISA Feline Reference value

来  源:   DOI:10.22099/IJVR.2023.46793.6721   PDF(Pubmed)

Abstract:
BACKGROUND: Optimal vitamin D levels for an effective role in immune function and rickets prevention are thought to vary, but have not yet been definitively determined. Reports on reference values of 25-hydroxyvitamin D (25(OH)D) in cats are limited.
OBJECTIVE: The study provides information about serum 25(OH)D values in cats with different age, gender, breed, diet type, reproductive status, housing condition, and also the relationship between these levels and various hematological and biochemical parameters.
METHODS: Clinically healthy cats (88) were included in the study. Physical examination and assessment of hematological and biochemical parameters were performed on cats in order to confirm their health status. Reference value of serum 25(OH)D was assayed by ELISA method and the effects of age (under six months and above six months), gender, breed, diet (only commercial diet, only homemade food, mixture of commercial and homemade food), reproduction status, and housing conditions on serum 25(OH)D was determined.
RESULTS: The median, 2.5% and 97.5% of 25(OH)D in sampled cats were 19.74 ng/ml, 3.12 ng/ml, and 92.1 ng/ml, respectively. Serum 25(OH)D concentration was lower when homemade diet was used compared to commercial and mixed diets as well as in cats under six months of age compared to older cats.
CONCLUSIONS: Diet type and age can affect serum 25(OH)D levels in healthy cats while other parameters had no significant effects.
摘要:
背景:认为在免疫功能和预防病方面发挥有效作用的最佳维生素D水平各不相同,但尚未确定。关于猫中25-羟基维生素D(25(OH)D)参考值的报道有限。
目的:该研究提供了不同年龄猫血清25(OH)D值的信息,性别,品种,饮食类型,生殖状态,住房条件,以及这些水平与各种血液学和生化参数之间的关系。
方法:临床健康的猫(88)包括在研究中。对猫进行身体检查以及血液学和生化参数的评估,以确认其健康状况。ELISA法检测血清25(OH)D的参考值及年龄(6个月以下及6个月以上)的影响,性别,品种,饮食(仅商业饮食,只有自制的食物,商业和自制食品的混合物),繁殖状态,并确定了血清25(OH)D的住房条件。
结果:中位数,2.5%和97.5%的25(OH)D采样猫为19.74ng/ml,3.12ng/ml,和92.1ng/ml,分别。与商业和混合饮食相比,使用自制饮食时的血清25(OH)D浓度较低,与年龄较大的猫相比,六个月以下的猫的血清25(OH)D浓度较低。
结论:饮食类型和年龄可以影响健康猫的血清25(OH)D水平,而其他参数没有显着影响。
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