PDF(Pubmed)
Abstract：
This study investigated the presence and abundance of Cystoisospora suis oocysts in faecal samples from 131 one- to three-week-old pig litters belonging to eight intensively raised, indoor herds in Spain. Seven herds used preventive anticoccidial toltrazuril treatments administered orally or by intramuscular injection, and one did not use preventive anticoccidial treatments. The diagnosis was performed using two oocyst flotation-concentration methods, Bailenger\'s method in every herd and the more recent Joachim\'s method in four herds. Oocysts were detected in every farm, the proportion of oocyst-positive samples was higher with Bailenger\'s technique, and the estimated overall prevalence (95% confidence interval) was 40 (32-49)%, including 47 (29-65)% in non-medicated litters, 52 (38-67)% in orally medicated litters and 28 (16-40)% in intramuscularly medicated litters (p < 0.05). However, mixed logistic regression models indicated that the risk of infection was not significantly associated with preventive anticoccidial treatments (p > 0.05), and increased with age, was higher in herds with partially compared to fully slatted dung floors in farrowing pens and in litters with pigs with diarrhoea (p < 0.05). The median (range) oocysts per gram of faeces (OpG) in infected litters by Bailenger\'s method was 623 (35-49048) and mixed negative binomial models revealed no significant association between infection intensity in positive litters and pen\'s floor type and piglets age, faecal consistency and treatment status (p > 0.05). The apparent low efficacy of Toltrazuril suggests treatment administration failures, reduced residual efficacy or low susceptibility of C. suis strains in study farms and needs further investigation.
摘要：
这项研究调查了来自131个1至3周龄猪窝的粪便样本中猪囊孢子卵囊的存在和丰度，这些猪窝属于8个密集饲养，室内牧群在西班牙。七个牛群使用了口服或肌肉注射的预防性抗球虫托曲唑治疗，一个人没有使用预防性抗球虫治疗。使用两种卵囊浮选浓缩方法进行诊断，Bailenger的方法在每个牛群中，而最近的Joachim的方法在四个牛群中。每个农场都检测到卵囊，使用Bailenger的技术，卵囊阳性样本的比例更高，估计的总体患病率（95％置信区间）为40（32-49）％，包括47（29-65）％的非药物垃圾，口服含药窝为52（38-67）％，肌内含药窝为28（16-40）％（p<0.05）。然而,混合logistic回归模型显示,预防性抗球虫治疗与感染风险无显著相关性(p>0.05),随着年龄的增长，与分娩围栏中的完全板条粪便地板和腹泻猪的垃圾相比，部分牛群的粪便地板更高（p<0.05）。通过Bailenger方法，感染的窝中每克粪便（OpG）的中位数（范围）卵囊为623（35-49048），混合阴性二项模型显示阳性窝的感染强度与围栏的地板类型和仔猪年龄之间没有显着关联，粪便稠度和治疗状态(p>0.05)。托曲天英的明显低疗效表明治疗失败，研究农场中猪链球菌菌株的残留功效降低或敏感性低，需要进一步调查。
