关键词: Arctic Bdellovibrio Svalbard biocrust predatory bacteria

Mesh : Svalbard Bdellovibrio Phylogeny RNA, Ribosomal, 16S / genetics Base Composition Sequence Analysis, DNA DNA, Bacterial / genetics Bacterial Typing Techniques Fatty Acids / chemistry Norway

来  源:   DOI:10.1099/ijsem.0.006248

Abstract:
Identified as a newly described species from a biocrust in Svalbard, Norway (78° 54\' 8.27″ N 12° 01\' 20.34″ E), isolate PAP01T has different characteristics from any known predatory bacteria. The isolate was vibrio-shaped strain that employed flagellar motility. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate clustered within the genus Bdellovibrio in the family Bdellovibrionaceae. 16S rRNA gene sequence similarities between strain PAP01T and the type strain (Bdellovibrio bacteriovorus HD100) was 95.7 %. The PAP01T genome has a size of 3.898 Mbp and possesses 3732 genes and a G+C content of 45.7 mol%. The results of genetic and physiological tests indicated the phenotypic differentiation of strain PAP01T from the two other Bdellovibrio species with validly published names. Based on the physiological and phylogenetic data, as well as the prey range spectrum and osmolality sensitivities, isolate PAP01T represents a novel species within the genus Bdellovibrio, for which the name Bdellovibrio svalbardensis sp. nov. is proposed. The type strain is PAP01T (=KCTC 92583T=DSM 115080T).
摘要:
被鉴定为斯瓦尔巴群岛生物科斯特的新描述物种,挪威(东经78°54\'8.27英寸北纬12°01\'20.34英寸),分离株PAP01T具有与任何已知的捕食性细菌不同的特性。分离物是弧菌状菌株,具有鞭毛运动性。基于16SrRNA基因序列的系统发育分析表明,该分离株聚集在Bdellovibrionaceae家族的Bdellovibrio属中。菌株PAP01T与类型菌株(BdellovibriobacteriovorusHD100)之间的16SrRNA基因序列相似性为95.7%。PAP01T基因组的大小为3.898Mbp,拥有3732个基因,GC含量为45.7mol%。遗传和生理测试结果表明,菌株PAP01T与其他两个具有有效出版名称的Bdellovibrio物种的表型分化。根据生理和系统发育数据,以及猎物范围谱和渗透压敏感性,分离株PAP01T代表了Bdellovibrio属中的一种新物种,其名称为Bdellovibriosvalbardensissp.11月。是提议的。菌株类型为PAP01T(=KCTC92583T=DSM115080T)。
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