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Abstract：
Sanghuangporus, also known as \"Sanghuang\" in China, is a well-known genus of traditional Chinese medicinal macrofungi. To make more effective use of Sanghuangporus resources, we completed the first genome assembly and annotation of a monokaryon strain of S. weigelae in the present study. A 33.96-Mb genome sequence was assembled as 13 contigs, leading to prediction of 9377 protein-coding genes. Phylogenetic and average nucleotide identity analyses indicated that the S. weigelae genome is closely related to those of other Sanghuangporus species in evolutionary tree, which clustered in one clade. Collinearity analysis revealed a high level of collinearity of S. weigelae with S. baumii, S. vaninii, and S. sanghuang. Biosynthesis pathways potentially involved in medicinal properties, including terpenoid and polysaccharide synthesis, were identified in S. weigelae, while polysaccharides were identified as the main medicinal metabolites in S. weigelae, with flavonoids more important in Sanghuangporus than other medicinal mushroom groups. Genes encoding 332 carbohydrate-active enzymes were identified in the S. weigelae genome, including major glycoside hydrolases and glycosyltransferases predicted, revealing the robust lignocellulose degradation capacity of S. weigelae. Further, 130 genes, clustered in seven classes were annotated to encode cytochromes P450 in the S. weigelae genome. Overall, our results reveal the remarkably medicinal capacity of S. weigelae and provide new insights that will inform the study of evolution and medicinal application of S. weigelae. The data are a reference resource for the formulation of scientific and rational ecological protection policies for Sanghuangporus species.
摘要：
三黄porus,在中国也被称为“桑黄”，是著名的中药大型真菌属。为了更有效地利用三黄孢菌资源,在本研究中，我们完成了S.weigelae单核菌株的第一个基因组组装和注释。一个33.96-Mb基因组序列被组装为13个重叠群，导致9377个蛋白质编码基因的预测。系统发育和平均核苷酸同一性分析表明，在进化树中，WeigelaeS.聚集在一个分支中。共线性分析显示Weigelae与鲍氏链球菌具有高度共线性，S.Vaninii,还有S.sanghang.可能涉及药用特性的生物合成途径，包括萜类和多糖的合成，在S.Weigelae中被发现，虽然多糖被确定为S.weigelae的主要药用代谢产物，黄酮类化合物比其他药用蘑菇组更重要。在S.weigelae基因组中鉴定出编码332种碳水化合物活性酶的基因，包括预测的主要糖苷水解酶和糖基转移酶，揭示了S.weigelae强大的木质纤维素降解能力。Further,130个基因,在S.weigelae基因组中编码细胞色素P450的七类注释。总的来说,我们的研究结果揭示了S.weigelae的显着药用能力，并提供了新的见解，将为S.weigelae的进化和药用应用研究提供信息。这些数据是制定科学合理的三黄孢菌生态保护政策的参考资源。
