PDF(Pubmed)
Abstract：
BACKGROUND: Universal stress proteins (USPs) are a class of stress-induced proteins that play a crucial role in biotic and abiotic stress responses. These proteins have previously been reported to participate directly in responses to various stress and protect plants against unfavorable environmental conditions. However, there is limited research on USPs in cotton, and systematic characterization of USPs in Gossypium species is lacking.
RESULTS: In the present study, the USP genes in Gossypium hirsutum were systematically identified and classified into six distinct subfamilies. The expansion of USPs in Gossypium species is mainly caused by dispersed duplication and whole genome duplication. Notably, the USPs that have expanded through allotetraploidization events are highly conserved in the allotetraploid species. The promoter regions of GhUSPs contain a diverse range of cis-acting elements associated with stress response. The RNA-Seq analysis and RT-qPCR assays revealed a significant induction of numerous GhUSPs expressions in response to various abiotic stresses. The co-expression network of GhUSPs revealed their involvement in stress response.
CONCLUSIONS: This study systematically analyzed the biological characteristics of GhUSPs and their response to abiotic stress. These findings serve as a theoretical basis for facilitating the breeding of cotton varieties in future research.
RESULTS: In the present study, the USP genes in Gossypium hirsutum were systematically identified and classified into six distinct subfamilies. The expansion of USPs in Gossypium species is mainly caused by dispersed duplication and whole genome duplication. Notably, the USPs that have expanded through allotetraploidization events are highly conserved in the allotetraploid species. The promoter regions of GhUSPs contain a diverse range of cis-acting elements associated with stress response. The RNA-Seq analysis and RT-qPCR assays revealed a significant induction of numerous GhUSPs expressions in response to various abiotic stresses. The co-expression network of GhUSPs revealed their involvement in stress response.
CONCLUSIONS: This study systematically analyzed the biological characteristics of GhUSPs and their response to abiotic stress. These findings serve as a theoretical basis for facilitating the breeding of cotton varieties in future research.
摘要：
背景：通用应激蛋白（USP）是一类应激诱导的蛋白质，在生物和非生物应激反应中起关键作用。先前已报道这些蛋白质直接参与对各种胁迫的响应并保护植物免受不利环境条件的影响。然而,对棉花USP的研究有限，并且缺乏对棉属物种USP的系统表征。
结果：在本研究中，系统鉴定了陆地棉的USP基因,并将其分为6个不同的亚家族.棉属物种中USP的扩展主要是由分散复制和全基因组复制引起的。值得注意的是,通过异源四倍体化事件扩展的USP在异源四倍体物种中高度保守。GhUSP的启动子区域含有与应激反应相关的各种顺式作用元件。RNA-Seq分析和RT-qPCR测定揭示了响应于各种非生物胁迫的大量GhUSP表达的显著诱导。GhUSPs的共表达网络揭示了它们参与应激反应。
结论：本研究系统分析了GhUSPs的生物学特性及其对非生物胁迫的响应。这些发现为今后棉花品种选育提供了理论依据。
结果：在本研究中，系统鉴定了陆地棉的USP基因,并将其分为6个不同的亚家族.棉属物种中USP的扩展主要是由分散复制和全基因组复制引起的。值得注意的是,通过异源四倍体化事件扩展的USP在异源四倍体物种中高度保守。GhUSP的启动子区域含有与应激反应相关的各种顺式作用元件。RNA-Seq分析和RT-qPCR测定揭示了响应于各种非生物胁迫的大量GhUSP表达的显著诱导。GhUSPs的共表达网络揭示了它们参与应激反应。
结论：本研究系统分析了GhUSPs的生物学特性及其对非生物胁迫的响应。这些发现为今后棉花品种选育提供了理论依据。
