Abstract：
Cystic echinococcosis (CE) is a common zoonotic disease caused by the larval form of Echinococcus granulosus sensu lato. This study determined the genotype and haplotype differences using the NADH dehydrogenase subunit 5 gene in hydatid cyst samples. Human (n = 12), cattle (n = 28), and sheep (n = 31) hydatid cyst isolates were included. Seventy-one genomic DNA samples were successfully extracted, and a 759 bp mitochondrial NADH dehydrogenase subunit 5 gene fragment was amplified by PCR. Following the sequence analysis, E. granulosus sensu stricto isolates were identified as G1 (n = 61) and G3 (n = 10). A total of 23 haplotypes were obtained from the 71 E. granulosus s.s. G1 and G3 samples. The main haplotype was Hap01 (60.56 %), which consisted of the G1 genotype. The second largest haplotype was Hap04, which consisted entirely of the G3 genotype. Hap14 acted as a bridge between the G1 and G3 genotypes. This study identifies G1 as the dominant genotype in humans and farm animals in Turkey. High haplotype and nucleotide diversity in genotypes were observed. Additionally, this is the first report on the phylogeography and gene flow models of the E. granulosus s.s. population in Turkey using the NADH dehydrogenase subunit 5 gene, the best marker distinguishing between G1 and G3 genotypes.
摘要：
囊性包虫病（CE）是一种常见的人畜共患疾病，由细粒棘球蚴的幼虫形式引起。这项研究使用NADH脱氢酶亚基5基因确定了包虫囊肿样品中的基因型和单倍型差异。人类（n=12），牛（n=28），包括绵羊（n=31）包虫囊肿分离株。成功提取了71个基因组DNA样本，并通过PCR扩增759bp的线粒体NADH脱氢酶亚基5基因片段。在序列分析之后，分离株为G1(n=61)和G3(n=10)。从71个细粒大肠杆菌s.s.G1和G3样品中获得总共23个单倍型。主要单倍型为Hap01(60.56%),由G1基因型组成。第二大单倍型是Hap04,其完全由G3基因型组成。Hap14充当G1和G3基因型之间的桥梁。这项研究确定G1为土耳其人类和农场动物的优势基因型。观察到基因型的高单倍型和核苷酸多样性。此外,这是第一次报告E.granulosuss.s.使用NADH脱氢酶亚基5基因在土耳其的种群的系统地理学和基因流模型，区分G1和G3基因型的最佳标记。
