PDF(Pubmed)
Abstract：
Objective: Umbilical cord-derived mesenchymal stem cell membrane-loaded minoxidil (MXD) nanoparticles (STCM-MXD-NPs) were prepared to investigate their effects on hair growth in C57BL/6J mice. Methods: STCM-MXD-NPs were obtained by freeze-thawing and differential centrifugation, and their effects on hair growth were evaluated using C57BL/6J mice. The mRNA and protein expression levels of vascular endothelial growth factor (VEGF) and insulin-like growth factor-1 (IGF-1) were detected by real-time polymerase chain reaction and enzyme-linked immunosorbent assays, respectively. Protein expression levels of marker of proliferation Ki-67 (MKI67) and β-catenin (CTNNB) in skin tissue were detected by immunohistochemistry. Results: STCM-MXD-NPs improved MXD solubility. They released the drug slowly, increasing its transdermal properties, accumulation in the skin, and content in the hair bulb tissues with a better efficacy than that of ordinary MXD. Moreover, STCM-MXD-NPs significantly upregulated the mRNA and protein levels of VEGF and IGF-1 and promoted the protein expression of MKI67 and CTNNB in mouse skin tissues, promoting mouse hair growth. Conclusion: Stem cell membrane-loaded MXD nanoparticles with slow-release properties increased MXD accumulation in the skin by improving its transdermal properties, increasing VEGF, IGF-1, MKI67, and CTNNB expression levels and promoting hair growth in C57BL/6J mice.
摘要：
目的:制备脐带间充质干细胞膜载米诺地尔(MXD)纳米粒(STCM-MXD-NPs),研究其对C57BL/6J小鼠毛发生长的影响。方法:采用冻融和差速离心法获得STCM-MXD-NPs,用C57BL/6J小鼠评价其对毛发生长的影响。通过实时聚合酶链反应和酶联免疫吸附试验检测血管内皮生长因子(VEGF)和胰岛素样生长因子-1(IGF-1)的mRNA和蛋白表达水平,分别。免疫组化法检测皮肤组织中增殖标志物Ki-67（MKI67）和β-catenin（CTNNB）的蛋白表达水平。结果:STCM-MXD-NP提高了MXD溶解度。他们慢慢释放了药物，增加其透皮特性，积聚在皮肤上，而在毛球组织中的含量具有比普通MXD更好的功效。此外，STCM-MXD-NPs显著上调小鼠皮肤组织中VEGF和IGF-1的mRNA和蛋白水平,促进MKI67和CTNNB的蛋白表达,促进小鼠毛发生长。结论:负载干细胞膜的MXD纳米粒具有缓释特性,通过改善MXD在皮肤中的透皮特性,增加VEGF，在C57BL/6J小鼠中IGF-1、MKI67和CTNNB表达水平和促进毛发生长。
