PDF(Pubmed)
Abstract：
This article provides the results of a study of the interaction of placental growth factor with adipose-derived stem cells (ASCs) of various origins, as well as the possibility of generating osteoplastic material based on xenogeneic matrix functionalization with human placental growth factor (PLGF). It is demonstrated that the greatest release of this factor from the functionalized material into the medium occurs during the first 3 h of contact with the model medium, but then the levels of the factor being released fall sharply, although release did continue throughout the 7 days of observation. The modified material was not cytotoxic, and its surface provided good cell adhesion. During 3 days of cultivation, the ASCs proliferated and migrated more actively on the surfaces of the modified material than on the surfaces of the control material. This study can serve as the basis for the development of original methods to functionalize such osteoplastic material by increasing PLGF immobilization by creating stronger bonds in order to regulate both factor dosage and the dynamics of the factor release into the environment. Further studies in experimental animals should facilitate assessment of the effectiveness of the functionalized materials. Such studies will be useful in the development of osteoplastic materials with new properties resulting from the inclusion of growth factors and in research on their biological activity.
摘要：
本文提供了胎盘生长因子与各种来源的脂肪干细胞（ASCs）相互作用的研究结果，以及基于人胎盘生长因子（PLGF）的异种基质功能化产生成骨材料的可能性。证明了该因子从功能化材料到介质中的最大释放发生在与模型介质接触的前3小时内，但是释放的因子水平急剧下降，虽然释放确实持续了整个7天的观察。改性材料没有细胞毒性，它的表面提供了良好的细胞粘附。在3天的培养过程中，ASC在改性材料的表面上比在对照材料的表面上更活跃地增殖和迁移。这项研究可以作为开发原始方法的基础，该方法通过创建更强的键来增加PLGF的固定来功能化这种骨形成材料，以调节因子剂量和因子释放到环境中的动力学。对实验动物的进一步研究应有助于评估功能化材料的有效性。这些研究将有助于开发具有由于包含生长因子而产生的新特性的骨形成材料以及对其生物活性的研究。
