关键词: Bacillus anthracis Bacillus cereus sensu stricto FTIR-spectroscopy IR Biotyper

来  源:   DOI:10.3390/microorganisms12010183   PDF(Pubmed)

Abstract:
Fourier transform infrared spectroscopy (FTIRS) is a diagnostic technique historically used in the microbiological field for the characterization of bacterial strains in relation to the specific composition of their lipid, protein, and polysaccharide components. For each bacterial strain, it is possible to obtain a unique absorption spectrum that represents the fingerprint obtained based on the components of the outer cell membrane. In this study, FTIRS was applied for the first time as an experimental diagnostic tool for the discrimination of two pathogenic species belonging to the Bacillus cereus group, Bacillus anthracis and Bacillus cereus sensu stricto; these are two closely related species that are not so easy to differentiate using classical microbiological methods, representing an innovative technology in the field of animal health.
摘要:
傅里叶变换红外光谱(FTIRS)是一种诊断技术,历史上用于微生物领域,用于表征细菌菌株与其脂质的特定组成有关,蛋白质,和多糖成分。对于每种细菌菌株,有可能获得代表基于外细胞膜成分获得的指纹的独特吸收光谱。在这项研究中,FTIRS首次被用作区分属于蜡状芽孢杆菌组的两种病原物种的实验诊断工具,炭疽芽孢杆菌和蜡状芽孢杆菌;这是两个密切相关的物种,使用经典的微生物学方法不太容易区分,代表动物健康领域的创新技术。
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