PDF(Pubmed)
Abstract：
Transcriptional variation has been studied but post-transcriptional modification due to RNA editing has not been investigated in Plasmodium. We investigated developmental stage-specific RNA editing in selected genes in Plasmodium falciparum 3D7. We detected extensive amination- and deamination-type RNA editing at 8, 16, 24, 32, 40, and 46 h in tightly synchronized Plasmodium. Most of the editing events were observed in 8 and 16 h ring-stage parasites. Extensive A-to-G deamination-type editing was detected more during the 16 h ring stage (25%) than the 8 h ring stage (20%). Extensive U-to-C amination-type editing was detected more during the 16 h ring stage (31%) than the 8 h ring stage (22%). In 28S, rRNA editing converted the loop structure to the stem structure. The hemoglobin binding activity of PF3D7_0216900 was also altered due to RNA editing. Among the expressed 28S rRNA genes, PF3D7_0532000 and PF3D7_0726000 expression was higher. Increased amounts of the transcripts of these two genes were found, particularly PF3D7_0726000 in the ring stage and PF3D7_0532000 in the trophozoite and schizont stages. Adenosine deaminase (ADA) expression did not correlate with the editing level. This first experimental report of RNA editing will help to identify the editing machinery that might be useful for antimalarial drug discovery and malaria control.
摘要：
已经研究了转录变异，但尚未在疟原虫中研究由于RNA编辑引起的转录后修饰。我们研究了恶性疟原虫3D7中选定基因的发育阶段特异性RNA编辑。我们在紧密同步的疟原虫中在8、16、24、32、40和46h检测到广泛的氨基和脱氨基类型的RNA编辑。大多数编辑事件在8和16h环期寄生虫中观察到。在16h环阶段（25％）比8h环阶段（20％）检测到更多的A到G脱氨类型编辑。在16h环阶段（31％）比8h环阶段（22％）检测到更多的U到C胺化类型编辑。在28S，rRNA编辑将环结构转化为茎结构。PF3D7_0216900的血红蛋白结合活性也由于RNA编辑而改变。在表达的28SrRNA基因中，PF3D7_0532000和PF3D7_0726000表达量较高。发现这两个基因的转录量增加，特别是环阶段的PF3D7_0726000和滋养体和裂殖体阶段的PF3D7_0532000。腺苷脱氨酶（ADA）表达与编辑水平无关。RNA编辑的第一个实验报告将有助于确定可能对抗疟药物发现和疟疾控制有用的编辑机制。
