PDF(Pubmed)
Abstract：
Members of the Anelloviridae family dominate the blood virome, emerging early in life. The anellome, representing the variety of anelloviruses within an individual, stabilizes by adulthood. Despite their supposedly commensal nature, elevated anellovirus concentrations under immunosuppressive treatment indicate an equilibrium controlled by immunity. Here, we investigated whether anelloviruses are sensitive to the immune activation that accompanies a secondary infection. As a model, we investigated 19 health care workers (HCWs) with initial SARS-CoV-2 infection, with blood sampling performed pre and post infection every 4 weeks in a 3-month-follow-up during the early 2020 COVID-19 pandemic. A concurrently followed control group (n = 27) remained SARS-CoV-2-negative. Serum anellovirus loads were measured using qPCR. A significant decrease in anellovirus load was found in the first weeks after SARS-CoV-2 infection, whereas anellovirus concentrations remained stable in the uninfected control group. A restored anellovirus load was seen approximately 10 weeks after SARS-CoV-2 infection. For five subjects, an in-time anellome analysis via Illumina sequencing could be performed. In three of the five HCWs, the anellome visibly changed during SARS-CoV-2 infection and returned to baseline in two of these cases. In conclusion, anellovirus loads in blood can temporarily decrease upon an acute secondary infection.
摘要：
在血病毒科中,在生命的早期出现。Anellome，代表个体内的各种anellovirus，成年后稳定下来。尽管它们被认为是共生的，免疫抑制治疗下的anellovirus浓度升高表明免疫控制的平衡。这里,我们调查了anellovirus是否对伴随继发感染的免疫激活敏感.作为一个模型,我们调查了19名最初感染SARS-CoV-2的医护人员（HCWs），在2020年初的COVID-19大流行期间，在3个月的随访中，每4周进行一次感染前后的血液采样。同时随访的对照组（n=27）保持SARS-CoV-2阴性。使用qPCR测量血清anellovirus载量。在SARS-CoV-2感染后的最初几周发现了anellovirus载量的显着下降，而在未感染的对照组中，anellovirus浓度保持稳定。SARS-CoV-2感染后约10周，可以看到恢复的anellovirus载量。对于五个科目，可以通过Illumina测序进行及时的分析。在五个HCW中的三个中，在SARS-CoV-2感染期间,Anellome发生了明显的变化,其中2例恢复到基线.总之,急性继发感染时，血液中的anellovirus载量可以暂时降低。
