PDF(Pubmed)
Abstract：
Elephantopus scaber L. (ESL) is a Chinese herb that is used both as a food and medicine, often being added to soups in summer in south China to relieve heat stress (HS), but its exact mechanism of action is unknown. In this study, heat-stressed mice were gavaged with ESL polysaccharides (ESLP) at 0, 150, 300, and 450 mg/kg/d-1 (n = 5) for seven days. The gut microbiota composition, short-chain fatty acids (SCFAs), seven neurotransmitters in faeces, expression of intestinal epithelial tight junction (TJ) proteins (Claudin-1, Occludin), and serum inflammatory cytokines were measured. The low dose of ESLP (ESLL) improved the adverse physiological conditions; significantly reduced the cytokines (TNF-α, IL-1β, IL-6) and lipopolysaccharide (LPS) levels (p < 0.05); upregulated the expression of Claudin-1; restored the gut microbiota composition including Achromobacter and Oscillospira, which were at similar levels to those in the normal control group; significantly increased beneficial SCFAs like butyric acid and 5-HT levels in the faeces of heat-stressed mice; and significantly decreased the valeric acid and glutamic acid level. The level of inflammatory markers significantly correlated with the above-mentioned indicators (p < 0.05). Thus, ESLL reduced the HS-induced systemic inflammation by optimizing gut microbiota (Achromobacter, Oscillospira) abundance, increasing gut beneficial SCFAs like butyric acid and 5-HT levels, and reducing gut valeric and glutamic acid levels.
摘要：
ElephantopusscaberL.（ESL）是一种中草药，既用作食品又用作药品，在中国南方的夏天经常被添加到汤中以缓解热应力(HS)，但其确切的作用机制是未知的。在这项研究中,热应激小鼠以0、150、300和450mg/kg/d-1（n=5）的剂量给药ESL多糖（ESLP）7天。肠道菌群组成，短链脂肪酸（SCFA），粪便中的七种神经递质,肠上皮紧密连接（TJ）蛋白（Claudin-1，Occludin）的表达，并测定血清炎性细胞因子。低剂量的ESLP(ESLL)改善了不利的生理状况;显著降低了细胞因子(TNF-α,IL-1β,IL-6）和脂多糖（LPS）水平（p<0.05）；上调Claudin-1的表达；恢复了肠道微生物群组成，包括无色杆菌属和蛇形螺旋体，与正常对照组的水平相似；显着增加了热应激小鼠粪便中有益的SCFA，如丁酸和5-HT水平；并显着降低了戊酸和谷氨酸水平。炎性标志物水平与上述指标显著相关(p<0.05)。因此，ESLL通过优化肠道微生物群来减少HS诱导的全身性炎症(无色杆菌，Oscillospira)丰度，增加肠道有益的SCFA，如丁酸和5-HT水平，降低肠戊酸和谷氨酸水平。
