PDF(Pubmed)
Abstract：
This hypothesis demonstrates that the efficiency of loop-mediated isothermal amplification (LAMP) for nucleic acid detection can be positively influenced by the preconcentration of microbial cells onto hydrophobic paper surfaces. The mechanism of this model is based on the high affinity of microbes towards hydrophobic surfaces. Extensive studies have demonstrated that hydrophobic surfaces exhibit enhanced bacterial and fungal adhesion. By exploiting this inherent affinity of hydrophobic paper substrates, the preconcentration approach enables the adherence of a greater number of target cells, resulting in a higher concentration of target templates for amplification directly from urine samples. In contrast to conventional methods, which often involve complex procedures, this approach offers a simpler, cost-effective, and user-friendly alternative. Moreover, the integration of cell adhesion, LAMP amplification, and signal readout within paper origami-based devices can provide a portable, robust, and highly efficient platform for rapid nucleic acid detection. This innovative hypothesis holds significant potential for point-of-care (POC) diagnostics and field surveillance applications. Further research and development in this field will advance the implementation of this technology, contributing to improved healthcare systems and public health outcomes.
摘要：
该假设表明，核酸检测的环介导等温扩增（LAMP）的效率可能会受到微生物细胞在疏水性纸表面上的预浓缩的积极影响。该模型的机制基于微生物对疏水表面的高亲和力。广泛的研究已经证明疏水表面表现出增强的细菌和真菌粘附。通过利用疏水性纸张基材的这种固有亲和力，预浓缩方法可以粘附更多的靶细胞，导致更高浓度的目标模板用于直接从尿液样品扩增。与传统方法相比，通常涉及复杂的程序，这种方法提供了一个更简单的，成本效益高,和用户友好的替代方案。此外，细胞粘附的整合，LAMP扩增,和基于折纸的设备内的信号读出可以提供便携式，健壮,和高效的快速核酸检测平台。这种创新的假设对于即时（POC）诊断和现场监视应用具有巨大的潜力。该领域的进一步研究和发展将推动该技术的实施，有助于改善医疗保健系统和公共卫生结果。
