PDF(Pubmed)
Abstract：
Keratin-related proteins (KAPs) are structural components of wool fibers and are thought to play a key role in regulating the physical and mechanical properties of fibers. Among all KAP genes (KRTAPs), KRTAP6 gene family (KRTAP6-1, KRTAP6-2, KRTAP6-3, KRTAP6-4, and KRTAP6-5) is a very important member with high polymorphism and notable association with some wool traits. In this study, we used real-time fluorescence quantitative PCR (RT-qPCR) and in situ hybridization to investigate spatiotemporal expression of KRTAP6s. The results revealed that KRTAP6 family genes were significantly expressed during anagen compared to other stages (p < 0.05). And it was found the five genes were expressed predominantly in the dermal papillae, inner and outer root sheaths, and showed a distinct spatiotemporal expression pattern. Also, it was found that KRTAP6-1 and KRTAP6-5 mRNA expression was negatively correlated with wool mean fiber diameter (MFD) and mean staple strength (MSS) (p < 0.05). In summary, the KRTAP6 family genes share a similar spatiotemporal expression pattern. And KRTAP6-1 and KRTAP6-5 may regulate the MFD and MSS of Gansu Alpine fine-wool sheep wool by changing the expression.
摘要：
角蛋白相关蛋白（KAP）是羊毛纤维的结构成分，被认为在调节纤维的物理和机械性能中起关键作用。在所有KAP基因（KRTAP）中，KRTAP6基因家族（KRTAP6-1，KRTAP6-2，KRTAP6-3，KRTAP6-4和KRTAP6-5）是一个非常重要的成员，具有很高的多态性，与某些羊毛性状密切相关。在这项研究中,我们使用实时荧光定量PCR（RT-qPCR）和原位杂交技术研究了KRTAP6s的时空表达。结果表明，与其他阶段相比，KRTAP6家族基因在生长期期间显着表达（p<0.05）。发现这五个基因主要在真皮乳头中表达，内部和外部根鞘，并表现出明显的时空表达模式。此外，发现KRTAP6-1和KRTAP6-5mRNA表达与羊毛平均纤维直径（MFD）和平均短纤维强度（MSS）呈负相关（p<0.05）。总之,KRTAP6家族基因具有相似的时空表达模式。KRTAP6-1和KRTAP6-5可能通过改变表达来调控甘肃高寒细羊毛的MFD和MSS。
