PDF(Pubmed)
Abstract：
Chlorantraniliprole (CAP) is a new type of diamide insecticide that is mainly used to control lepidopteran pests. However, it has been proven to be hazardous to nontarget organisms, and the effects of its residues need to be monitored. In this study, five hybridoma cell lines were developed that produced anti-CAP monoclonal antibodies (mAbs), of which the mAb originating from the cell line 5C5B9 showed the highest sensitivity and was used to develop a gold nanoparticle-based lateral flow immunoassay (AuNP-LFIA) for CAP. The visible limit of detection of the AuNP-LFIA was 1.25 ng/mL, and the detection results were obtained in less than 10 min. The AuNP-LFIA showed no cross-reactivity for CAP analogs, except for tetraniliprole (50%) and cyclaniliprole (5%). In the detection of spiked and blind samples, the accuracy and reliability of the AuNP-LFIA were confirmed by a comparison with spiked concentrations and verified by ultra-performance liquid chromatography-tandem mass spectrometry. Thus, this study provides the core reagents for establishing CAP immunoassays and a AuNP-LFIA for the detection of residual CAP.
摘要：
Chloantraniliprole（CAP）是一种新型的二酰胺杀虫剂，主要用于控制鳞翅目害虫。然而,它已被证明对非目标生物有害，并且需要监测其残留物的影响。在这项研究中,开发了五种产生抗CAP单克隆抗体（mAb）的杂交瘤细胞系，其中源自细胞系5C5B9的mAb显示出最高的灵敏度，并用于开发基于金纳米颗粒的CAP侧流免疫测定（AuNP-LFIA）。AuNP-LFIA的可见检测限为1.25ng/mL，在10min内获得检测结果。AuNP-LFIA对CAP类似物无交叉反应性，除了tetraniliprole（50％）和cyclaniliprole（5％）。在加标和盲样品的检测中，AuNP-LFIA的准确性和可靠性通过与加标浓度的比较得到证实,并通过超高效液相色谱-串联质谱进行验证.因此，本研究为建立CAP免疫测定法和检测残留CAP的AuNP-LFIA提供了核心试剂。
