PDF(Pubmed)
Abstract：
As an ancient and endangered species unique to the Yangtze River in China, the wild population of the Dabry\'s sturgeon has become scarce. Due to the long time till the first sexual maturity of Dabry\'s sturgeon, the population of artificially bred Dabry\'s sturgeon recovered slowly. As a member of the tachykinin family, TAC1 has been reported to have a variety of functions in mammals such as pain control, smooth muscle contraction and reproductive cycle regulation, but the function of Tac1 in fish has been rarely reported. In this study, we synthesized two tac1 gene products, Substance P (SP) and neurokinin A (NKA), and further verified the effect of two tac1 gene products on the secretion of related hormones in the pituitary of Dabry\'s Sturgeon by intraperitoneal injection and co-culture of primary cells. Expression studies revealed that the newly cloned tac1 were mainly distributed in the hypothalamus and pituitary tissue of the brain. In prepubertal Dabry\'s sturgeon, this study showed that the two gonadotropins\' mRNA levels in pituitary tissue can be significantly increased by SP and NKA through intraperitoneal injection, and the LH protein level in serum was also increased. Further study showed that both NKA and SP could promote the two gonadotropins\' mRNA expression in pituitary cells of Dabry\'s sturgeon. In addition, we explored the optimal dose and time of SP and NKA on pituitary cells is 24 h and over 10 nM. These results, as a whole, suggested that tac1 gene products play an important role in gonadotropin release and gonadal development in prepubertal Dabry\'s sturgeon.
摘要：
作为中国长江特有的古老濒危物种，Dabry的st鱼的野生种群已经变得稀缺。由于达布瑞st的第一次性成熟的时间很长，人工繁殖的达布瑞的st鱼种群恢复缓慢。作为速激肽家族的一员,据报道，TAC1在哺乳动物中具有多种功能，例如控制疼痛，平滑肌收缩和生殖周期调节，但Tac1在鱼类中的功能却鲜有报道。在这项研究中,我们合成了两种tac1基因产物,P物质（SP）和神经激肽A（NKA），并通过腹腔注射和原代细胞共培养进一步验证了两种tac1基因产物对Dabry鱼垂体相关激素分泌的影响。表达研究表明，新克隆的tac1主要分布在大脑的下丘脑和垂体组织中。在青春期前Dabry\的st鱼中，这项研究表明，腹膜内注射SP和NKA可显著增加垂体组织中两种促性腺激素的mRNA水平，血清LH蛋白水平也升高。进一步的研究表明，NKA和SP均能促进达布氏st垂体细胞中两种促性腺激素的mRNA表达。此外,我们探索了SP和NKA对垂体细胞的最佳剂量和时间是24小时和超过10nM。这些结果,作为一个整体,提示tac1基因产物在青春期前Dabryst鱼的促性腺激素释放和性腺发育中起重要作用。
