PDF(Pubmed)
Abstract：
In modern aquaculture, genomics-driven breeding programs have emerged as powerful tools for optimizing fish quality. This study focused on two emblematic Mediterranean fish species, the European seabass (Dicentrarchus labrax) and the gilthead sea bream (Sparus aurata), with a primary aim of exploring the genetic basis of white muscle/fillet degradation in fresh fish following harvest. We identified 57 and 44 missense SNPs in gilthead sea bream and European seabass, respectively, located within genes encoding for endogenous proteases responsible for fillet quality. These SNPs were cherry-picked based on their strategic location within the catalytic/regulatory domains of endogenous proteases that are expressed in the white muscle. Using MassArray technology, we successfully associated differentiated enzymatic activity of those endogenous proteases post-harvest as a phenotypic trait with genetic polymorphism of six SNPs in gilthead sea bream and nine in European seabass. These findings can be valuable attributes in selective breeding programs toward the extension of freshness and shelf life of these species. The integration of MassArray technology into breeding programs offers a cost-effective strategy for harnessing the potential of these genetic variants to enhance the overall quality of the final product. Recognizing that fresh fish perishability is a challenge, extending shelf-life is pivotal in reducing losses and production costs.
摘要：
在现代水产养殖中,基因组学驱动的育种计划已经成为优化鱼类质量的强大工具。这项研究集中在两种具有象征意义的地中海鱼类上，欧洲海底鲈鱼（Dicentrarchuslabrax）和金头海鱼（Sparusaurata），主要目的是探索收获后鲜鱼白肌/鱼片降解的遗传基础。我们在金头鲈鱼和欧洲鲈鱼中确定了57和44个错误的SNP，分别,位于编码负责鱼片质量的内源性蛋白酶的基因内。这些SNP是基于它们在白肌肉中表达的内源性蛋白酶的催化/调节结构域内的战略位置而挑选的。使用MassArray技术，我们成功地将收获后这些内源性蛋白酶的分化酶活性作为表型性状与金头鱼中的六个SNP和欧洲鲈鱼中的九个SNP的遗传多态性相关联。这些发现在延长这些物种的新鲜度和保质期的选择性育种计划中可能是有价值的属性。将MassArray技术整合到育种计划中提供了一种具有成本效益的策略，可以利用这些遗传变异的潜力来提高最终产品的整体质量。认识到鲜鱼易腐性是一个挑战，延长保质期是减少损失和生产成本的关键。
