PDF(Pubmed)
Abstract：
BACKGROUND: Lameness is defined as altered or abnormal gait due to dysfunction of the locomotor system, and is a health issue of feedlot cattle, having major economic, labour, and welfare implications. Digital dermatitis (DD-a lesion of the plantar surface of the foot) and foot rot (FR-affects the interdigital cleft) are common infectious causes of lameness in feedlots. These hoof lesions can occur alone or in combination (DD + FR) in the same hoof. A total of 208 hoof swabs were collected from three commercial feedlots located in southern Alberta. Every lesion sample was matched with a corresponding control skin sample taken from a healthy contralateral foot. Control skin samples were also collected from cattle with no lesion on any feet. Bacterial communities of three types of hoof lesions (DD, DD + FR, FR) and healthy skin were profiled using 16S amplicon sequencing.
RESULTS: Alpha diversity analysis revealed a lower bacterial diversity on DD and FR lesions compared to control skin. Beta diversity analysis showed that bacterial communities of DD, FR, and DD + FR lesions were distinct from those of the control skin. While the impact of feedlot was minimal, lesion type contributed to 22% of the variation observed among bacterial communities (PERMANOVA-R = 0.22, P < 0.01). Compared to the corresponding control skin, there were 11, 12, and 3 differentially abundant (DA) bacterial genera in DD, DD + FR, and FR lesions, respectively.
CONCLUSIONS: The bacterial community description of a DD + FR lesion is a novel finding. Not only did lesions lead to altered bacterial communities when compared to healthy skin, but the composition of those communities also differed depending on the hoof lesion. The 16S amplicon sequencing of surface swabs has significant value as a research tool in separating different hoof lesions and can provide additional insights to the polybacterial etiology of DD and FR in feedlot cattle.
RESULTS: Alpha diversity analysis revealed a lower bacterial diversity on DD and FR lesions compared to control skin. Beta diversity analysis showed that bacterial communities of DD, FR, and DD + FR lesions were distinct from those of the control skin. While the impact of feedlot was minimal, lesion type contributed to 22% of the variation observed among bacterial communities (PERMANOVA-R = 0.22, P < 0.01). Compared to the corresponding control skin, there were 11, 12, and 3 differentially abundant (DA) bacterial genera in DD, DD + FR, and FR lesions, respectively.
CONCLUSIONS: The bacterial community description of a DD + FR lesion is a novel finding. Not only did lesions lead to altered bacterial communities when compared to healthy skin, but the composition of those communities also differed depending on the hoof lesion. The 16S amplicon sequencing of surface swabs has significant value as a research tool in separating different hoof lesions and can provide additional insights to the polybacterial etiology of DD and FR in feedlot cattle.
摘要：
背景：跛行被定义为由于运动系统功能障碍而改变或异常的步态，是饲养场牛的健康问题，有重大的经济，劳动，和福利影响。数字皮炎（足足底表面的DD-病变）和脚腐烂（FR-影响指间裂隙）是饲养场跛行的常见感染原因。这些蹄病变可以单独或组合（DDFR）在同一蹄中发生。从位于艾伯塔省南部的三个商业饲养场总共收集了208个蹄拭子。每个损伤样本与取自健康对侧足部的相应对照皮肤样本相匹配。还从任何脚上没有损伤的牛收集对照皮肤样品。三种蹄病变的细菌群落（DD，DD+FR,FR)和健康皮肤使用16S扩增子测序进行轮廓分析。
结果：Alpha多样性分析显示，与对照皮肤相比，DD和FR病变上的细菌多样性较低。β多样性分析表明,DD的细菌群落,FR,DD+FR病变与对照皮肤不同。虽然饲养场的影响很小，病变类型占细菌群落变化的22％（PERMANOVA-R=0.22，P<0.01）。与相应的对照皮肤相比，DD中有11、12和3个差异丰富（DA）细菌属，DD+FR,和FR病变，分别。
结论：DD+FR病变的细菌群落描述是一个新发现。与健康皮肤相比，病变不仅导致细菌群落改变，但是这些群落的组成也因蹄部病变而异。表面拭子的16S扩增子测序作为分离不同蹄病变的研究工具具有重要价值，并且可以为饲养场牛DD和FR的多细菌病因提供更多见解。
结果：Alpha多样性分析显示，与对照皮肤相比，DD和FR病变上的细菌多样性较低。β多样性分析表明,DD的细菌群落,FR,DD+FR病变与对照皮肤不同。虽然饲养场的影响很小，病变类型占细菌群落变化的22％（PERMANOVA-R=0.22，P<0.01）。与相应的对照皮肤相比，DD中有11、12和3个差异丰富（DA）细菌属，DD+FR,和FR病变，分别。
结论：DD+FR病变的细菌群落描述是一个新发现。与健康皮肤相比，病变不仅导致细菌群落改变，但是这些群落的组成也因蹄部病变而异。表面拭子的16S扩增子测序作为分离不同蹄病变的研究工具具有重要价值，并且可以为饲养场牛DD和FR的多细菌病因提供更多见解。
