RESULTS: For genetic association analysis we included MYD88 and TLR4, PRRs sensing proteins. Allele distribution for MYD88 rs6853 (A > G) and TLR4 rs7873784 (C > G) presented conserved among the tested samples with statistically differential distribution in TB patients versus controls. However, when testing according to sample ethnicity (African or Caucasian-derived individuals) we identified that the rs6853 G/G genotype was associated with a lower susceptibility to TB in Caucasian population. Meanwhile, the rs7873784 G/G genotype was associated with a higher TB susceptibility in Afro-descendant ethnicity individuals. We also aimed to verify MYD88 and the inflammasome genes NLRP1 and NLRC4 expression in order to connect to active TB and/or clinical aspects.
CONCLUSIONS: We identified that inflammasome gene expression in TB patients under treatment display a similar pattern as in healthy controls, indicating that TB treatment impairs NLRP1 inflammasome activation.
结果:对于遗传关联分析,我们包括MYD88和TLR4,PRRs传感蛋白。MYD88rs6853(A>G)和TLR4rs7873784(C>G)的等位基因分布在测试样品中保守,在TB患者与对照组中具有统计学差异分布。然而,在根据样本种族(非洲或白种人来源的个体)进行检测时,我们发现rs6853G/G基因型与白种人人群对TB的易感性较低相关.同时,rs7873784G/G基因型与非洲裔种族个体中更高的TB易感性相关.我们还旨在验证MYD88和炎症体基因NLRP1和NLRC4的表达,以便与活动性结核病和/或临床方面联系起来。
结论:我们发现,在接受治疗的结核病患者中,炎性体基因表达与健康对照相似。表明结核病治疗损害NLRP1炎性体激活。