PDF(Pubmed)
Abstract：
In response to the urgent requirement for rapid, precise, and cost-effective detection in intensive care units (ICUs) for ventilated patients, as well as the need to overcome the limitations of traditional detection methods, researchers have turned their attention towards advancing novel technologies. Among these, biosensors have emerged as a reliable platform for achieving accurate and early diagnoses. In this study, we explore the possibility of using Pyocyanin analysis for early detection of pathogens in ventilator-associated pneumonia (VAP) and lower respiratory tract infections in ventilated patients. To achieve this, we developed an electrochemical sensor utilizing a graphene oxide-copper oxide-doped MgO (GO - Cu - Mgo) (GCM) catalyst for Pyocyanin detection. Pyocyanin is a virulence factor in the phenazine group that is produced by Pseudomonas aeruginosa strains, leading to infections such as pneumonia, urinary tract infections, and cystic fibrosis. We additionally investigated the use of DNA aptamers for detecting Pyocyanin as a biomarker of Pseudomonas aeruginosa, a common causative agent of VAP. The results of this study indicated that electrochemical detection of Pyocyanin using a GCM catalyst shows promising potential for various applications, including clinical diagnostics and drug discovery.
摘要：
为了应对快速的迫切要求，精确，以及在重症监护病房（ICU）中对通气患者进行具有成本效益的检测，以及需要克服传统检测方法的局限性，研究人员已经把注意力转向推进新技术。其中,生物传感器已成为实现准确和早期诊断的可靠平台。在这项研究中,我们探讨了在呼吸机相关性肺炎(VAP)和通气患者的下呼吸道感染中使用Pypalyin分析早期检测病原体的可能性.为了实现这一点，我们开发了一种利用氧化石墨烯-氧化铜掺杂MgO（GO-Cu-Mgo）（GCM）催化剂的电化学传感器，用于检测氰化素。氰化素是吩嗪组中由铜绿假单胞菌菌株产生的一种毒力因子,导致肺炎等感染，尿路感染,囊性纤维化.我们还研究了使用DNA适体检测作为绿脓杆菌的生物标志物的花青素，VAP的常见致病因子。这项研究的结果表明，使用GCM催化剂对Pymicroin进行电化学检测显示出各种应用的潜力，包括临床诊断和药物发现。
