PDF(Pubmed)
Abstract：
This study investigates a real-time handheld bioaerosol monitoring system for the detection of biological particles using UV-LED and light-induced fluorescence technology. Biological particles produce both scattering and fluorescence signals simultaneously, which can help distinguish them from general particles. The detected scattering, fluorescence, and simultaneous signals are then converted into photon signals and categorized based on predetermined criteria. A reliable biological particle generator was required to validate the performance of the system. This study explores the use of an M13 bacteriophage as a virus simulant of biological agents and employs a customized inkjet aerosol generator to produce M13 bacteriophage aerosols of a specific size by controlling the concentration of M13. We confirmed that micro-sized, narrowly dispersed M13 aerosols were efficiently generated. Additionally, we confirmed the performance of this real-time handheld bioaerosol monitoring system by detecting viruses.
摘要：
本研究研究了一种使用UV-LED和光诱导荧光技术检测生物颗粒的实时手持式生物气溶胶监测系统。生物粒子同时产生散射和荧光信号,这有助于将它们与一般粒子区分开来。检测到的散射，荧光，然后将同时信号转换成光子信号并根据预定标准进行分类。需要可靠的生物粒子发生器来验证系统的性能。这项研究探索了使用M13噬菌体作为生物制剂的病毒模拟物，并采用定制的喷墨气溶胶发生器通过控制M13的浓度来生产特定大小的M13噬菌体气溶胶。我们证实了微型，有效地产生了狭窄分散的M13气溶胶。此外,我们通过检测病毒证实了这种实时手持生物气溶胶监测系统的性能。
