RESULTS: Both Rosin and Rosetax-21 were tested under simulated clean and dirty conditions (with BSA) against the ESKAPE pathogens, and C. difficile spore preparations. In clean conditions, Rosin (5% weight/volume: w/v) demonstrated significant efficacy against five of the ESKAPE pathogens, with A. baumannii and E. faecium being the most susceptible, and K. pneumoniae the most resistant, showing only a one-log reduction after a 5 min treatment. However, in dirty conditions, all pathogens including K. pneumoniae exhibited at least a 3-log reduction to Rosin within 5 min. Rosetax-21 (5% w/v) was found to be less effective than Rosin in clean conditions, a trend that was exacerbated in the presence of BSA. Additionally, both Rosin and Rosetax-21 at 2.5% (w/v) achieved complete eradication of C. difficile spores when combined with 0.5% glutaraldehyde, though their standalone sporicidal activity was limited.
CONCLUSIONS: The findings from this study highlight the potential of Rosin and Rosetax-21 as both bactericidal and sporicidal disinfectants, with their efficacy varying based on the conditions and the pathogens tested. This presents an avenue for the development of novel healthcare disinfection strategies, especially against HAIs caused by antimicrobial-resistant ESKAPE pathogens and C. difficile.
结果:松香和Rosetax-21均在模拟清洁和肮脏条件下(使用BSA)对ESKAPE病原体进行了测试,和艰难梭菌孢子制剂。在清洁条件下,松香(5%重量/体积:w/v)显示出对五种ESKAPE病原体的显着功效,鲍曼不动杆菌和屎肠杆菌最易感,肺炎克雷伯菌的耐药性最强,在5分钟的治疗后仅显示一个对数的减少。然而,在肮脏的条件下,包括肺炎克雷伯菌在内的所有病原体在5分钟内显示出至少3-log的松香减少。在清洁条件下,Rosetax-21(5%w/v)的效果不如松香,在BSA的存在下加剧了这一趋势。此外,当与0.5%戊二醛结合时,2.5%(w/v)的松香和Rosetax-21均完全根除艰难梭菌孢子,尽管它们独立的杀孢子活性有限。
结论:这项研究的发现强调了松香和Rosetax-21作为杀菌和杀孢子消毒剂的潜力,其功效根据所测试的条件和病原体而有所不同。这为开发新的医疗保健消毒策略提供了途径,特别是针对由抗菌素耐药性ESKAPE病原体和艰难梭菌引起的HAIs。