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Abstract：
The Rocahepevirus ratti hepatitis E virus genotype C1 (HEV-C1) has been documented to infect humans. However, the understanding of HEV-C1 remains constrained. This study aims to determine the prevalence and genomic characteristics of HEV-C1 in small animals in Yunnan province of southwestern China. A total of 444 liver tissues were collected from animals covering the orders Rodentia, Soricomorpha, Scandentia and Erinaceomorpha in three regions in Yunnan. Then Paslahepevirus balayani and Rocahepevirus were examined using RT-qPCR. The detection rate of Rocahepevirus was 12.95 % (36/278) in animals of order Rodentia, with 14.77 % (35/237) in Rattus tanezumi and 33.33 % (1/3) in Niviventer fulvescens. No Paslahepevirus balayani was detected. Additionally, two full-length Rocahepevirus sequences (MSE-17 and LHK-54) and thirty-three partial ORF1 sequences were amplified and determined to be HEV-C1. MSE-17 and LHK-54 shared moderate nucleotide identity (78.9 %-80.3 %) with HEV-C1 isolated in rats and humans. The HEV-C1 isolated from Niviventer fulvescens demonstrated a 100 % nucleotide identity with that from Rattus tanezumi. The rat HEV-C1 sequences isolated in our study and other Asian HEV-C1 sequences were phylogenetically distant from those isolated in North America and Europe. Furthermore, the two full-length sequences isolated in our study had less amino acid substitutions in the motifs of RNA-dependent RNA polymerase domain (F204L and L238F), compared with other Asian sequences. In summary, HEV-C1 commonly spreads in rats in Yunnan province of China. Our findings suggest a spatially associated phylogeny, and potential cross-species transmission of HEV-C1.
摘要：
Rocahepevirusratti戊型肝炎病毒基因型C1（HEV-C1）已被证明感染人类。然而,对HEV-C1的理解仍然受到限制。本研究旨在确定中国西南部云南省小动物中HEV-C1的患病率和基因组特征。总共从动物中收集了444个肝脏组织，涵盖了啮齿动物，索利科莫，云南三个地区的斯堪的亚和赤霉病。然后使用RT-qPCR检查巴勒亚尼Paslahepevirusbalayani和Rocahepevirus。Rodentia类动物的Rocahepe病毒检出率为12.95％（36/278），家兔中14.77%(35/237)，Niviventer足月中33.33%(1/3)。未检测到Paslahepevirusbalayani。此外,扩增了两个全长Rocahepevirus序列（MSE-17和LHK-54）和33个ORF1部分序列，并确定为HEV-C1。MSE-17和LHK-54与在大鼠和人类中分离的HEV-C1共享中等核苷酸同一性(78.9%-80.3%)。从NiviventerFulvescens中分离出的HEV-C1与Rattustanezumi具有100％的核苷酸同一性。在我们的研究中分离的大鼠HEV-C1序列和其他亚洲HEV-C1序列在系统发育上与在北美和欧洲分离的序列相距遥远。此外，在我们的研究中分离的两个全长序列在RNA依赖性RNA聚合酶结构域（F204L和L238F）的基序中具有较少的氨基酸取代，与其他亚洲序列相比。总之,HEV-C1通常在中国云南省的大鼠中传播。我们的发现表明空间相关的系统发育，以及HEV-C1的潜在跨物种传播。
