PDF(Pubmed)
Abstract：
BACKGROUND: Anthrax is a severe zoonotic infectious disease caused by Bacillus anthracis. Most reported cases were traditionally diagnosed through culture and microscopy. We reported here the second case of cutaneous anthrax diagnosed by metagenomic next-generation sequencing (mNGS).
METHODS: A 63-year-old man had a history of contact with an unwell sheep, developing local redness and swelling on wrist. The dorsal side of the left hand and forearm, with tension blisters on the back of the left.
METHODS: B anthracis was detected from culturing and mNGS of tension blisters.
METHODS: On the second day of admission, the patient was administered 3.2 million units of penicillin every 6 hours, and isolated and closely observed.
RESULTS: The patient improves and is discharged.
CONCLUSIONS: Traditional bacterial cultures are time-consuming, while mNGS offers the advantage of accurate, quick, high-throughput, unbiased sequencing of all genetic material in a sample, which is a good technical tool for assisting in the diagnosis of rare pathogen infections.
METHODS: A 63-year-old man had a history of contact with an unwell sheep, developing local redness and swelling on wrist. The dorsal side of the left hand and forearm, with tension blisters on the back of the left.
METHODS: B anthracis was detected from culturing and mNGS of tension blisters.
METHODS: On the second day of admission, the patient was administered 3.2 million units of penicillin every 6 hours, and isolated and closely observed.
RESULTS: The patient improves and is discharged.
CONCLUSIONS: Traditional bacterial cultures are time-consuming, while mNGS offers the advantage of accurate, quick, high-throughput, unbiased sequencing of all genetic material in a sample, which is a good technical tool for assisting in the diagnosis of rare pathogen infections.
摘要：
背景：炭疽是由炭疽芽孢杆菌引起的一种严重的人畜共患传染病。大多数报告的病例传统上是通过培养和显微镜诊断的。我们在这里报告了通过宏基因组下一代测序（mNGS）诊断的第二例皮肤炭疽病。
方法：一名63岁的男子有与不适绵羊接触的病史，手腕出现局部红肿。左手和前臂的背侧，左侧后部有张力水泡。
方法：从张力水泡的培养和mNGS中检测到B炭疽病。
方法：在入院的第二天，患者每6小时服用320万单位青霉素，孤立和密切观察。
结果：患者好转出院。
结论：传统的细菌培养非常耗时，虽然MNGS提供了准确的优势，快,高通量，样本中所有遗传物质的无偏测序，这是辅助诊断罕见病原体感染的良好技术工具。
方法：一名63岁的男子有与不适绵羊接触的病史，手腕出现局部红肿。左手和前臂的背侧，左侧后部有张力水泡。
方法：从张力水泡的培养和mNGS中检测到B炭疽病。
方法：在入院的第二天，患者每6小时服用320万单位青霉素，孤立和密切观察。
结果：患者好转出院。
结论：传统的细菌培养非常耗时，虽然MNGS提供了准确的优势，快,高通量，样本中所有遗传物质的无偏测序，这是辅助诊断罕见病原体感染的良好技术工具。
