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Abstract：
The restricted migration evaluation is conducive to more complex tumor migration research because of the conformity with in vivo tumors. However, the differences between restricted and unrestricted cell migration and the distinction between different evaluation methods have not been systematically studied, hindering related research. In this study, by constructing the restricted environments on chips, the influence of co-culture conditions on the cancer cell migration capacity was studied. The results showed that the restricted channels can discriminate the influence of weak tumor environmental factors on complex tumor migration behaviors by limiting the free growth instinct of tumor cells. Through the comparison of 2D and 3D restricted migration methods, the extracellular matrix (ECM) restriction was also helpful in distinguishing the influence of the weak tumor environmental factor. However, the 3D ECM can better reflect the tortuosity of the cell migration process and the cooperative behavior among cancer cells. In the anticancer drug evaluation, 3D ECM can more accurately reflect the cytotoxicity of drugs and is more consistent with the drug resistance in the human body. In conclusion, the research will help to distinguish different evaluation methods of cancer cell migration, help researchers select appropriate evaluation models, and promote the research of tumor metastasis.
摘要：
限制性迁移评价由于与体内肿瘤相符，有利于更复杂的肿瘤迁移研究。然而,限制性和非限制性细胞迁移之间的差异以及不同评估方法之间的区别尚未得到系统研究，阻碍相关研究。在这项研究中,通过在芯片上构建受限环境，研究了共培养条件对癌细胞迁移能力的影响。结果表明，受限通道可以通过限制肿瘤细胞的自由生长本能来区分弱肿瘤环境因素对复杂肿瘤迁移行为的影响。通过二维和三维限制迁移方法的比较，细胞外基质（ECM）限制也有助于区分弱肿瘤环境因素的影响。然而,3DECM可以更好地反映细胞迁移过程的曲折和癌细胞之间的合作行为。在抗癌药物评价中，3DECM可以更准确地反映药物的细胞毒性，更符合人体内的耐药性。总之,这项研究将有助于区分癌细胞迁移的不同评估方法，帮助研究人员选择合适的评估模型，促进肿瘤转移的研究。
