PDF(Pubmed)
Abstract：
Staphylococcus coagulans (SC) belongs to a group of coagulase-positive staphylococci occasionally isolated from the skin lesions of dogs with pyoderma. We recently revealed that erythritol, a sugar alcohol, inhibited the growth of SC strain JCM7470. This study investigated the molecular mechanisms involved in this growth inhibition of JCM7470 by erythritol, and determine whether erythritol inhibits the growth of SC isolated from the skin of dogs with pyoderma. Comprehensive analysis of the gene expression of JCM7470 in the presence of erythritol revealed that erythritol upregulated the expression of glcB and ptsG genes, both of which encode phosphotransferase system (PTS) glucoside- and glucose-specific permease C, B, and A domains (EIICBA), respectively, associated with sugar uptake. Moreover, erythritol suppressed in vitro growth of all 27 SC strains isolated from the skin lesions of canine pyoderma, including 13 mecA gene-positive and 14 mecA gene-negative strains. Finally, the growth inhibition of the SC clinical isolates by erythritol was restored by the addition of glucose. In summary, we revealed that erythritol promotes PTS gene expression and suppresses the in vitro growth of SC clinical isolates from dogs with pyoderma. Restoration of the erythritol-induced growth inhibition by glucose suggested that glucose starvation may contribute to the growth inhibition of SC.
摘要：
凝固葡萄球菌（SC）属于一组凝固酶阳性葡萄球菌，偶尔从脓皮病犬的皮肤病变中分离出来。我们最近发现赤藓糖醇,一种糖醇，抑制SC菌株JCM7470的生长。这项研究调查了赤藓糖醇对JCM7470生长抑制的分子机制，并确定赤藓糖醇是否抑制从脓皮病犬皮肤分离的SC的生长。在赤藓糖醇存在下对JCM7470基因表达的综合分析显示，赤藓糖醇上调glcB和ptsG基因的表达，两者都编码磷酸转移酶系统（PTS）葡糖苷和葡萄糖特异性通透酶C，B,和A域（EIICBA），分别,与糖吸收有关。此外，赤藓糖醇抑制了从犬脓皮病皮肤病变中分离出的所有27种SC菌株的体外生长，其中mecA基因阳性13株,mecA基因阴性14株。最后,添加葡萄糖可恢复赤藓糖醇对SC临床分离株的生长抑制作用.总之,我们发现赤藓糖醇促进PTS基因表达并抑制脓皮病犬SC临床分离株的体外生长。葡萄糖对赤藓糖醇诱导的生长抑制的恢复表明，葡萄糖饥饿可能有助于SC的生长抑制。
